|
| Status |
Public on Aug 19, 2017 |
| Title |
20435 Low [P1750_3] |
| Sample type |
RNA |
| |
|
| Source name |
pancreas tumor macrophage MHCIIl Low
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: FvBn
genotype/variation: Not provided
tissue: pancreas tumor
cell type: macrophage MHCIIl Low
gender: female
age: 8-10 weeks
|
| Treatment protocol |
Animals were implanted with tumors and cells were isolated by FACS directly into RNA Lysis buffer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using ENZO total RNA kit.
|
| Label |
Cy5
|
| Label protocol |
cDNAs were amplified using the Sigma-WTA2 kit, and chemically labeled with Kreatech ULS RNA labeling kit (Kreatech Diagnostics) and Cy5-labeled cDNAs were hybridized to Agilent Mouse Gene Expression Microarrays either 8x60K (G4852B-07809) or 4x44K (G4846A-026655).
|
| |
|
| Hybridization protocol |
Hybridization of the labeled cDNAs was done in Agilent 2x gene expression hybridization buffer, Agilent 10x blocking reagent and kreatech Kreablock onto Agilent microarrays at 65oC for 20 min.
|
| Scan protocol |
Slides were scanned on an Agilent C-class Microarray scanner.
|
| Description |
P1750_3
|
| Data processing |
Gridding and analysis of images was performed using Agilent Feature Extraction V11.5.1.1.
Intensity values were Feature Extraction processed signal values (called rProcessedSignal) in log2 scale. Control probes were filtered off and duplicate probes were averaged before quantile-normalization. There are 4 data sets, and each of them was normalized by the quantile method separately.
|
| |
|
| Submission date |
Aug 18, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
DAvid DeNardo |
| E-mail(s) |
ddenardo@wustl.edu
|
| Organization name |
Washington University
|
| Department |
Medicine
|
| Street address |
660 S. Euclid Ave
|
| City |
St. Louis |
| State/province |
Mo |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL21163 |
| Series (2) |
| GSE102833 |
Expression profiling macrophage subsets [P1750] |
| GSE102836 |
Expression profiling macrophage subsets |
|
