|
| Status |
Public on Mar 01, 2018 |
| Title |
CKCS_Saliva_Unaffected_5 |
| Sample type |
genomic |
| |
|
| Source name |
Saliva
|
| Organism |
Canis lupus familiaris |
| Characteristics |
breed: Cavalier King Charles Spaniel
disease state: control
sm: 0
ccd size: 0
vd: 1
f-diam: 46.51
ab: 15.81
bc: 17.84
cd: 13.92
bd: 29.06
ae: 32.89
ID: 29.81
ac: 29.64
ai: 29.11
cj: 12.28
fh: 27.89
fg: 22.05
bk: 10.7
l1 (hae): 118.73
l2 (fac): 73.63
l3 (dib): 72.2
l4 (fae): 44.19
l5 (aeb): 28.49
l6(dba): 103.21
l7(dbi): 26.28
l8 (ebd): 10.75
l9 (jcb): 50.62
l10 (afg): 50.97
l11(ebk): 52.79
f-d/cd: 3.341235632
f-d/bc: 2.60706278
l4+l7: 70.47
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from blood or saliva using the ORAGENE Animal kit (DNAgenotek)
|
| Label |
CY3 and CY5
|
| Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37¡C using amplification master mix (WG-MA1, WG-MA2) and primer/neutralization mix (WG-MSM). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FMS), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
|
| |
|
| Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48¡C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
| Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a iScan Autoloader 2X (Illumina).
|
| Description |
11404-D0002558-WG3318316-DNA-A05
|
| Data processing |
Genomic DNA extracted from saliva was genotyped using Infinium CanineHD Genotyping BeadChips (Illumina).
GenomeStudio 2.0 was used to generate processed data using the final report function.
|
| |
|
| Submission date |
Aug 22, 2017 |
| Last update date |
Mar 01, 2018 |
| Contact name |
Philippe Lemay |
| E-mail(s) |
lemayph@gmail.com
|
| Organization name |
Université de Montréal
|
| Street address |
3175 Chemin de la Côte-Sainte-Catherine
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3T 1C5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17481 |
| Series (1) |
| GSE102906 |
Whole genome QTL study identifies candidate loci associated to syringomyelia |
|
