|
| Status |
Public on Mar 01, 2018 |
| Title |
CKCS_Saliva_Unaffected_83 |
| Sample type |
genomic |
| |
|
| Source name |
Saliva
|
| Organism |
Canis lupus familiaris |
| Characteristics |
breed: Cavalier King Charles Spaniel
disease state: control
sm: 0
ccd size: 0
vd: 1
f-diam: 49.1
ab: 16.37
bc: 20.01
cd: 12.73
bd: 30.17
ae: 35.74
ID: 31.15
ac: 31.83
ai: 31.89
cj: 12.61
fh: 28.48
fg: 0
bk: 15.02
l1 (hae): 121.07
l2 (fac): 69.69
l3 (dib): 72.73
l4 (fae): 43.59
l5 (aeb): 27.51
l6(dba): 102.33
l7(dbi): 29.35
l8 (ebd): 9.24
l9 (jcb): 48.87
l10 (afg): 0
l11(ebk): 28.48
f-d/cd: 3.857030636
f-d/bc: 2.453773113
l4+l7: 72.94
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from blood or saliva using the ORAGENE Animal kit (DNAgenotek)
|
| Label |
CY3 and CY5
|
| Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37¡C using amplification master mix (WG-MA1, WG-MA2) and primer/neutralization mix (WG-MSM). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FMS), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
|
| |
|
| Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48¡C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
| Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a iScan Autoloader 2X (Illumina).
|
| Description |
11404-D0003306-WG3318316-DNA-G11
|
| Data processing |
Genomic DNA extracted from saliva was genotyped using Infinium CanineHD Genotyping BeadChips (Illumina).
GenomeStudio 2.0 was used to generate processed data using the final report function.
|
| |
|
| Submission date |
Aug 22, 2017 |
| Last update date |
Mar 01, 2018 |
| Contact name |
Philippe Lemay |
| E-mail(s) |
lemayph@gmail.com
|
| Organization name |
Université de Montréal
|
| Street address |
3175 Chemin de la Côte-Sainte-Catherine
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3T 1C5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17481 |
| Series (1) |
| GSE102906 |
Whole genome QTL study identifies candidate loci associated to syringomyelia |
|
