|
| Status |
Public on Jul 01, 2021 |
| Title |
Patient 5 N5 |
| Sample type |
RNA |
| |
|
| Source name |
primary esophageal tissue, normal
|
| Organism |
Homo sapiens |
| Characteristics |
disease status: esophageal carcinoma
tissue: normal adjacent tissue
age: 63
gender: male
patient: 5
|
| Treatment protocol |
After grinded under the ice, the tissues were collected for further total RNA extraction.
|
| Growth protocol |
Esophageal carcinoma tissues and normal adjacent tissue were excised and stored at -80°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen) according to manufacturer’s instructions. RNA quality and quantity were measured by using NanoDrop spectrophotometer (ND-1000, NanoDrop Technologies) and RNA Integrity was determined by gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
Circular RNAs were amplified and transcribed into fluorescent cRNA utilizing a random priming method (Arraystar Super RNA Labeling Kit; Arraystar).
|
| |
|
| Hybridization protocol |
The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array V2 (8x15K, Arraystar).
|
| Scan protocol |
The hybridized arrays were scanned using the Agilent Scanner G2505C.
|
| Description |
N5
Tissues obtained from the patients by esophagoscopy or surgery.
Matched normal adjacent tissue of Ca5.
|
| Data processing |
Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed using the R software limma package.
|
| |
|
| Submission date |
Aug 25, 2017 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Xiaohua Chen |
| E-mail(s) |
cxh0663@126.com
|
| Phone |
00-86-020-34859211
|
| Organization name |
Panyu Central Hospital
|
| Street address |
Fuyudong Road 8，Qiaonan street，Panyu
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
511400 |
| Country |
China |
| |
|
| Platform ID |
GPL21825 |
| Series (1) |
| GSE103104 |
Microarray expression profiling of circular RNAs in human esophageal carcinoma |
|
