|
Status |
Public on Aug 31, 2017 |
Title |
MLL_04027 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Bone marrow/Blood
|
Organism |
Homo sapiens |
Characteristics |
gender: female tissue: Bone marrow/Blood diagnosis: Chronic Lymphocytic Leukemia (CLL)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted on a Qiacube according to manufacturer's protocol
|
Label |
Cy5
|
Label protocol |
labeling was done with SureTag Complete DNA Labeling Kit according to manufacturer's protocol, Cy3 were used for reference sample and Cy5 for the test sample
|
|
|
Channel 2 |
Source name |
Agilent Euro Female
|
Organism |
Homo sapiens |
Characteristics |
control type: commercially available normal genomic DNA
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Catalog number for Agilent male control DNA: #5190-4370. Catalog number for Agilent female control DNA: #5190-4371.
|
Label |
Cy3
|
Label protocol |
labeling was done with SureTag Complete DNA Labeling Kit according to manufacturer's protocol, Cy3 were used for reference sample and Cy5 for the test sample
|
|
|
|
Hybridization protocol |
We used SurePrint G3 ISCA CGH+SNP Bundle, 4x180K according to manufacturer's protocol
|
Scan protocol |
Protocol CytoCGH_0209_4x_Mar14 (Read Only) was used with CytoGenomics v 2.9.1.0 on instrument Agilent G5761A SureScan Dx Microarray Scanner System
|
Data processing |
Unmodified Raw data provided as separate files from CytoGenomics v2.9.1.0. We have used LogRatio directly extracted from the feature extraction software (Agilent CytoGenomics).
|
|
|
Submission date |
Aug 30, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Torsten Haferlach |
Organization name |
MLL Munich leukemia laboratory
|
Street address |
Max-Lebsche-Platz 31
|
City |
München |
ZIP/Postal code |
81377 |
Country |
Germany |
|
|
Platform ID |
GPL16237 |
Series (1) |
GSE103306 |
Normal karyotype CLL show high rate of SF3B1 mutations and genomic arrays aberrations with impact on time to treatment |
|