Female Acute Myeloid Leukemia with t(10;11)(q22;q23) Undifferentiated AML, CD65 negative negative by RT-PCR for BCR/ABL, AML1/ETO, CBFbeta/MYH11, MLL-TD and FLT3-ITD
Extracted molecule
total RNA
Extraction protocol
Qiagen RNeasy Plus mini
Label
Cy5
Label protocol
Random 9mers pre-labeled with Cy5
Hybridization protocol
See Roche NimbleGen website for details and Selzer RR, Richmond TA, Pofahl NJ, Green RD, Eis PS, et al. (2005) Analysis of chromosome breakpoints in neuroblastoma at sub-kilobase resolution using fine-tiling oligonucleotide array CGH. Genes Chromosomes Cancer 44: 305-319.
Scan protocol
See Selzer RR, Richmond TA, Pofahl NJ, Green RD, Eis PS, et al. (2005) Analysis of chromosome breakpoints in neuroblastoma at sub-kilobase resolution using fine-tiling oligonucleotide array CGH. Genes Chromosomes Cancer 44: 305-319.
Description
All samples for microarray hybridization were processed at the Roche NimbleGen Service Laboratory.
Data processing
Raw data (.pair files) from the scanner were processed with Roche NimbleGen's version of the RMA algorithm (without background correction) in NimbleScan 2.3 software.
