|
| Status |
Public on Mar 07, 2011 |
| Title |
CBS 513.88 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
A. niger CBS 513.88 mycelium grown on a glucose medium
|
| Organism |
Aspergillus niger |
| Characteristics |
Strain: CBS 513.88
Growth: Exponential phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen RNeasy Mini Kit(QIAGEN Nordic, Ballerup, Denmark), according to the protocol for isolation of total RNA from plant and fungi
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Standard Affymetrix GeneChip Expression Analysis protocol
|
| Scan protocol |
The array was washed and stained using a GeneChip Fluidics Station FS-400, and scanned on an Agilent GeneArray Scanner 3000
|
| Description |
Strain: CBS 513.88, Growth: Exponential phase
|
| Data processing |
Affymetrix CEL-data files were preprocessed using the statistical language and environment R version 2.5.1. The probe intensities were normalised for background using the robust multiarray average (RMA) method using only perfect match (PM) probes. Normalization was performed subsequently using the quantiles algorithm. Gene expression values were calculated from the PM probes with the medianpolish summary method. All statistical preprocessing methods were used by invoking them through the affy package.
|
| |
|
| Submission date |
Mar 29, 2008 |
| Last update date |
Mar 07, 2011 |
| Contact name |
Mikael R Andersen |
| E-mail(s) |
mr@bio.dtu.dk
|
| Organization name |
Technical University of Denmark
|
| Department |
BioCentrum-DTU
|
| Lab |
Center for Microbial Biotechnology
|
| Street address |
Søltofts plads, bygn 223
|
| City |
Kgs. Lyngby |
| ZIP/Postal code |
2800 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL5975 |
| Series (1) |
| GSE10983 |
Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88 |
|
