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Platform GPL5975 Query DataSets for GPL5975
Status Public on Feb 10, 2008
Title [AsprgDTUa520520F] Custom Affymetrix Aspergillus Chip DTU_3Aspergillus_45K_v1.0
Technology type in situ oligonucleotide
Distribution custom-commercial
Organisms Aspergillus niger; Aspergillus oryzae; Aspergillus nidulans
Manufacturer Affymetrix
Manufacture protocol Initial probe-design was done using the OligoWiz 2.0 software (Nielsen, 2003, Wernersson, 2005) from CDS sequences of predicted ORFs from the genome sequences of A. nidulans FGSC A4 (Galagan, 2005), A. niger ATCC 1015 (Baker, 2006) and A. oryzae RIB40 (Machida, 2005). For each gene, a maximum of 11 non-overlapping perfect match probes were calculated using the OligoWiz standard scoring of cross-hybridization, melting temperature, folding, position preference and low complexity. A 3' position preference for the probes was included in the computations. The probes were designed separately for each genome. Pruning of the probe sequences to comply with Affymetrix recommendations was done by removing duplicate probe sequences and shortening probes that were not possible to synthesize in full length. Also included on the chip iss a number of Affymetrix standard controls, custom controls, an A. oryzae EST-collection (courtesy of Novozymes A/S) and probes for ORFs from the S. coelicolor A3(2) (Bentley, 2002) genome.
Catalog number AspergDTUa520520F
Support glass
Coating quartz
 
Description Type: CustomExpress. Feature size: 11-micron. Format: 49-7875
 
Contributor(s) Andersen MR, Wongsangnak W, Panagiotou G, Borodina I, Nielsen J
Submission date Oct 09, 2007
Last update date Mar 23, 2010
Contact name Mikael R Andersen
E-mail(s) mr@bio.dtu.dk
Organization name Technical University of Denmark
Department BioCentrum-DTU
Lab Center for Microbial Biotechnology
Street address Søltofts plads, bygn 223
City Kgs. Lyngby
ZIP/Postal code 2800
Country Denmark
 
Samples (176) GSM235475, GSM235476, GSM235477, GSM235478, GSM235479, GSM235481 
Series (14)
GSE9275 A tri-species Aspergillus array (nidulans arrays)
GSE9276 A tri-species Aspergillus array (niger arrays)
GSE9277 A tri-species Aspergillus array (oryzae arrays)

Data table header descriptions
ID
DESCRIPTION The type of sequence used for feature probe design
ORGANISM The organism source of the feature
Internal_ORF ORF/locus name of the feature, if available. Can be used for accession from the respective genome web pages
ORF GenBank accession
SPOT_ID spot identifier

Data table
ID DESCRIPTION ORGANISM Internal_ORF ORF SPOT_ID
23.ORF1_at CDS Streptomyces coelicolor Streptomyces coelicolor ORF
23.ORF2_at CDS Streptomyces coelicolor Streptomyces coelicolor ORF
23.ORF3_at CDS Streptomyces coelicolor Streptomyces coelicolor ORF
23.ORF4_at CDS Streptomyces coelicolor Streptomyces coelicolor ORF
AB164642_11059_at CDS Streptomyces coelicolor AB164642
AB164642_12228_at CDS Streptomyces coelicolor AB164642
AB164642_13346_at CDS Streptomyces coelicolor AB164642
AB164642_13498_at CDS Streptomyces coelicolor AB164642
AB164642_14751_at CDS Streptomyces coelicolor AB164642
AB164642_15497_at CDS Streptomyces coelicolor AB164642
AB164642_16915_at CDS Streptomyces coelicolor AB164642
AB164642_1767_at CDS Streptomyces coelicolor AB164642
AB164642_17798_at CDS Streptomyces coelicolor AB164642
AB164642_19898_at CDS Streptomyces coelicolor AB164642
AB164642_21201_at CDS Streptomyces coelicolor AB164642
AB164642_22243_at CDS Streptomyces coelicolor AB164642
AB164642_22665_at CDS Streptomyces coelicolor AB164642
AB164642_23589_at CDS Streptomyces coelicolor AB164642
AB164642_2380_at CDS Streptomyces coelicolor AB164642
AB164642_3322_at CDS Streptomyces coelicolor AB164642

Total number of rows: 43776

Table truncated, full table size 2742 Kbytes.




Download family Format
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Supplementary file Size Download File type/resource
GPL5975_AspergDTUa520520F.cdf.gz 7.0 Mb (ftp)(http) CDF

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