|
| Status |
Public on Sep 28, 2017 |
| Title |
M1.2 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
age: 6 Weeks Old
Sex: Female
strain: C57Bl/6
genotype: WT
tissue: spleen
cell type: CD4+ T cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Buffer RLT
|
| Label |
Refer to the NanoString nCounter Assay System Manual
|
| Label protocol |
Refer to the NanoString nCounter Assay System Manual.
|
| |
|
| Hybridization protocol |
Refer to the NanoString nCounter Assay System Manual.
|
| Scan protocol |
Refer to the NanoString nCounter Assay System Manual.
|
| Description |
Refer to the NanoString nCounter Assay System Manual
|
| Data processing |
The raw data were normalized using 6 housekeeping genes (Actb, Gapdh, Hprt, Ldha, Ms4a1 and Tbp). nSolver Analysis Software was used for normalization and we followed their internal protocol with geometric mean as normalization factor.
|
| |
|
| Submission date |
Sep 27, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL24057 |
| Series (2) |
| GSE104344 |
FoxP3 scanning mutagenesis reveals functional variegation and mild mutations with atypical autoimmune phenotypes [nanostring] |
| GSE104346 |
FoxP3 scanning mutagenesis reveals functional variegation and mild mutations with atypical autoimmune phenotypes |
|
