|
| Status |
Public on Apr 30, 2008 |
| Title |
WT ovaries (AGO2IP) |
| Sample type |
SRA |
| |
|
| Source name |
small RNAs isolated from AGO2 immunoprecipitate from ovaries
|
| Organism |
Drosophila melanogaster |
| Characteristics |
from 2-4 day old flies
|
| Treatment protocol |
tissues and cells were harvested on ice
|
| Growth protocol |
flies and Schneider cells were grown under standard conditions at 25 degrees.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
extraction of total RNA was with Trizol; extraction of small RNAs from immunoprecipitates was with Phenol/Chloroform; before adaptor ligation, small RNAs were PAGE isolated;
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
small RNA from immunoprecipitate
|
| Data processing |
the 5'end of the Solexa read corresponds to the small RNA's 5'end. The 3' linker was computationally removed at the end of all reads if possible; only sequences matching the Drosophila genome with 100% identity are reported with the exception of the AGO1 and AGO2 IP libraries from Schneider cells, where also seqeunces matching NCBI deposited seqeunces for the Flock House Virus with up to 3 mismatches are included.
|
| |
|
| Submission date |
Apr 07, 2008 |
| Last update date |
Jun 11, 2013 |
| Contact name |
Julius Brennecke |
| E-mail(s) |
brenneck@cshl.edu
|
| Organization name |
Cold Spring Harbor Labs
|
| Lab |
Greg Hannon
|
| Street address |
1 Bungtown Road
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platform ID |
GPL9058 |
| Series (1) |
| GSE11086 |
Deep sequencing of Drosophila melanogaster small RNAs |
|
| Relations |
| BioSample |
SAMN02195353 |
