|
| Status |
Public on Feb 16, 2018 |
| Title |
46A1-s3 |
| Sample type |
RNA |
| |
|
| Source name |
gdT-derived iPS
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
origin: gdT cell
method: Sendai virus
|
| Growth protocol |
iPSCs were cultured in StemFit medium on plates coated with laminin-511 E8 fragments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol reagent (Life Technologies) according to the manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng of total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip primeview human gene expression array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using Genechip Scanner 3000 7G system.
|
| Description |
gdT cells
|
| Data processing |
The data were analyzed using the Gene Spring GX 13.1.1 software program (Agilent Technologies). We used RMA as summarization algorithm and performed quantile normalizaion. Baseline transformation was not performed.
|
| |
|
| Submission date |
Oct 05, 2017 |
| Last update date |
Feb 16, 2018 |
| Contact name |
Michiyo Koyanagi-Aoi |
| E-mail(s) |
mkoyaoi@med.kobe-u.ac.jp
|
| Organization name |
Kobe University
|
| Street address |
Chuo-ku, Kusunoki-cho 7-5-1
|
| City |
Kobe City |
| ZIP/Postal code |
650-0017 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE104605 |
Gene expression data of gdT-derived iPSCs and validated iPS clone for pluritest |
|
