|
| Status |
Public on Oct 10, 2017 |
| Title |
Ad-Epas1(HIF-2a) infected primary mouse articular chondrocytes, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Ad-Epas1(HIF-2a) infected primary mouse articular chondrocytes
|
| Organism |
Mus musculus |
| Characteristics |
cell type: articular chondrocytes
strain: ICR
age: 5 days
genotype: WT
treatment: Ad-Epas1(HIF-2a) infected
|
| Treatment protocol |
Primary mouse articular chondrocytes were infected with null(control) or recombinant adenovirus expressing Hypoxia inducible factor-2 alpha (HIF-2α). The cells were harvested for total RNA extraction at 24 h post infection.
|
| Growth protocol |
Cultured were maintained at 37 °C in an humidified atmosphere containing 5% CO2 as a monolayer in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with 10% fetal bovine serum and antibiotics (penicillin G and streptomycin). 37 °C, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from mouse articular chondrocytes using a Purelink RNA mini kit (Ambion). The concentration, purity, and integrity of the extracted RNA were determined by spectrophotometry.
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was then fragmented and biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit
|
| |
|
| Hybridization protocol |
Approximately 5.5 μg of labeled DNA target was hybridized to the Affymetrix GeneChip Array at 45°C for 16hour
|
| Scan protocol |
Hybridized arrays were washed and stained on a GeneChip Fluidics Station 450 and scanned on a GCS3000 Scanner (Affymetrix).
|
| Description |
Gene expression data from Ad-Epas1(HIF-2a) infected primary mouse articular chondrocytes
|
| Data processing |
Array data export processing and analysis was performed using Affymetrix® GeneChip Command Console® Software (AGCC). Probe signals in the raw data were normalized by the RMA (Robust Multi-array Average)
|
| |
|
| Submission date |
Oct 10, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Yoonkyung Won |
| E-mail(s) |
ykwon@gist.ac.kr
|
| Organization name |
Gwangju Institute of Science and Technology
|
| Street address |
123 Cheomdangwagiro
|
| City |
Gwangju |
| ZIP/Postal code |
61005 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE104794 |
Hypoxia inducible factor-2 alpha (HIF-2α) overexpression effect on primary mouse articular chondrocytes |
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