|
| Status |
Public on Nov 21, 2017 |
| Title |
293T EV |
| Sample type |
SRA |
| |
|
| Source name |
cell line HEK293T
|
| Organism |
Homo sapiens |
| Characteristics |
strain: cell line
genotype: ELF4-/-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Peritoneal macrophages and 293T cells were prepared, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
Genome_build: mm8
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample ...
|
| |
|
| Submission date |
Nov 20, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
lili Cao |
| E-mail(s) |
caolili@bjmu.edu.cn
|
| Organization name |
Peking university health science center
|
| Street address |
Xueyuan road, haidian district
|
| City |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE107174 |
Whole genome RNA-seq analysis of Wild type or Elf4-/- peritoneal macrophages and whole genome RNA-seq analysis of 293T cells transfected with miR-221 or empty vector. |
|
| Relations |
| BioSample |
SAMN08045520 |
| SRA |
SRX3410335 |
