|
| Status |
Public on Jan 19, 2018 |
| Title |
CD19-3-total2 |
| Sample type |
RNA |
| |
|
| Source name |
CD19+ cells from tonsil 3
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: CD19+ cells sorted from tonsil tissue obtained during routine tonsillectomy
age: 3
gender: female
|
| Treatment protocol |
CD19+ B cells were sorted by magnetic-activated cell sorting (MACS) from tonsil tissue samples of three donors between three and nine years old. Briefly, a freshly prepared tonsillar cell suspension was prepared and enriched for CD19+ cells using CD19-beads and LS columns (both Miltenyi Biotec, Leiden, The Netherlands). For BL tumor tissues, immunoprecipitation of AGO2-containing RISC complexes was performed on fifty 10 uM tissue sections of two fresh-frozen primary BL tumors.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Micro/Mini Kit (Qiagen)
|
| Label |
Cy5
|
| Label protocol |
Cy5 labeled using 50-100ng total RNA in combination with the LowInput QuickAmp Labelling Kit, Cyanine 5 CTP Dye Pack and RNA Spike-In kit (all Agilent Technologies). Each sample was labeled and hybridized in duplicate.
|
| |
|
| Hybridization protocol |
Overnight hybridization at 65oC on Agilent-050524 SurePrint G3 Custom Human 8x60K according to the manufacturers instructions
|
| Scan protocol |
Agilent DNA Microarray Scanner, Agilent Feature Extraction software version 10.7.3.1.
|
| Description |
AGO2-RIP was performed on CD19+ B cells sorted by magnetic-activated cell sorting (MACS) using CD19-beads and LS columns
mRNA and lncRNA expression analysis_tonsil-3_total fraction_2
|
| Data processing |
GeneSpring GX 12.5 software (Agilent Technologies); quantile normalization without base line transformation.
|
| |
|
| Submission date |
Nov 24, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Agnieszka Dzikiewicz-Krawczyk |
| E-mail(s) |
krawczyk@man.poznan.pl
|
| Phone |
+48616579219
|
| Organization name |
Institute of Human Genetics, Polish Academy of Sciences
|
| Street address |
Strzeszynska 32
|
| City |
Poznan |
| ZIP/Postal code |
60-479 |
| Country |
Poland |
| |
|
| Platform ID |
GPL18641 |
| Series (1) |
| GSE107325 |
Microarray analysis to identify miRNA targetome of primary Burkitt lymphoma tumors in comparison to normal CD19+ B cells |
|
