|
Status |
Public on Apr 23, 2019 |
Title |
MBC-15 |
Sample type |
genomic |
|
|
Source name |
primary tumor
|
Organism |
Homo sapiens |
Characteristics |
tissue: breast tumor
|
Growth protocol |
primary formalin fixed parafffin embedded tissue
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DANN was extracted from macrodissected with DNAeasy blood and tissue kit (Qiagen)
|
Label |
biotin
|
Label protocol |
as given by the manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 7G.
|
Description |
hybridized to [OncoScan] Affymetrix OncoScan array
|
Data processing |
CEL files were generated after scanning by the Affymetrix GeneChip Command Console (AGCC). These CEL files were further processed using OncoScan Console software version 1.3.0.39 to produce OSCHP files.
|
|
|
Submission date |
Nov 27, 2017 |
Last update date |
Apr 23, 2019 |
Contact name |
Doris Steinemann |
E-mail(s) |
steinemann.doris@mh-hannover.de
|
Organization name |
Medizinische Hochschule Hannover
|
Department |
Human Genetics
|
Street address |
Carl-Neuberg-Straße 1
|
City |
Hannover |
ZIP/Postal code |
30625 |
Country |
Germany |
|
|
Platform ID |
GPL18602 |
Series (1) |
GSE107394 |
Genomic aberrations in metaplastic breast carcinomas and adenomyoepitheliomas |
|