|
| Status |
Public on Oct 01, 2008 |
| Title |
18-24hpa regenerating fin fold vs. control |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Total RNA from regenerating 18-24hpa fin fold
|
| Organism |
Danio rerio |
| Characteristics |
zebrafish larval fin fold
|
| Treatment protocol |
Tissues were collected on dryice.
|
| Growth protocol |
Incubated in the egg water at 28.5 degree for 2 days before amputation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Amino Allyl MessageAmp™ aRNA kit frim Ambion (performed by DNA CHIP Institute Inc. (Yokohama, Japan))
|
| |
|
| Channel 2 |
| Source name |
Total RNA from control fin fold corresponding to 18-24hpa
|
| Organism |
Danio rerio |
| Characteristics |
zebrafish larval fin fold
|
| Treatment protocol |
Tissues were collected on dryice.
|
| Growth protocol |
Incubated in the egg water at 28.5 degree for 2 days before amputation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl MessageAmp™ aRNA kit frim Ambion (performed by DNA CHIP Institute Inc. (Yokohama, Japan))
|
| |
|
| |
| Hybridization protocol |
According to manufacturer's instruction (performed by DNA CHIP Institute Inc. (Yokohama, Japan))
|
| Scan protocol |
performed by DNA CHIP Institute Inc. (Yokohama, Japan)
|
| Description |
Gene expression profiling of zebrafish larval fin-fold regeneration comparing amputated fin fold and uncut control
|
| Data processing |
Valid spots: Intensity>BG+2*SD and Ch1_Intensity+Ch2_Intensity>=1000→TRUE; Values were shown only for valid spots.
|
| |
|
| Submission date |
May 08, 2008 |
| Last update date |
Jul 25, 2008 |
| Contact name |
Atsushi Kawakami |
| E-mail(s) |
atkawaka@bio.titech.ac.jp
|
| Phone |
+81-45-924-5717
|
| Fax |
+81-45-924-5718
|
| Organization name |
Tokyo Institute of Technology
|
| Department |
Biological Information
|
| Street address |
4259 Nagatsuta, Midori-ku
|
| City |
Yokohama |
| ZIP/Postal code |
226-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL531 |
| Series (1) |
| GSE11391 |
Regenerating zebrafish fin fold vs. uncut control |
|
