|
Status |
Public on Dec 06, 2018 |
Title |
RNAseq_B_ZF108DNAJ1inWT_NophenoControl_rep1 |
Sample type |
SRA |
|
|
Source name |
leaf
|
Organism |
Arabidopsis thaliana |
Characteristics |
ecotype: Col-0 tissue: leaf genotype/variation: ZF108DNAJ1inWT
|
Growth protocol |
soil, 16 hour light, 8 hour dark cycle
|
Extracted molecule |
polyA RNA |
Extraction protocol |
2-3 leaves from individual plants (2-3 weeks old). Total RNA was extracted using TRIzol and Direct-zol RNA miniprep kit (Zymo, R2050) including in-column DNase treatment. TruSeq Stranded mRNA Library Prep Kit (Illumina)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Description |
polyA RNA
|
Data processing |
RNA-seq: 50bp single end reads were aligned using TopHat2 including the fr-firststrand option allowing for intron length of up to 10,000. bigWig tracks were generated using bamCoverage in DeepTools (https://deeptools.readthedocs.io/en/latest/). genome build: TAIR 10 RNAseq bigWig tracks are normalised reads (by mapped library size), and binned into 25bp or 50bp bins.
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|
|
Submission date |
Dec 21, 2017 |
Last update date |
Dec 06, 2018 |
Contact name |
C. Jake Harris |
E-mail(s) |
jake.harris88@gmail.com
|
Organization name |
UCLA
|
Department |
MCDB
|
Lab |
Jacobsen
|
Street address |
4045 Terasaki Life Sciences Building
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL21785 |
Series (1) |
GSE108414 |
A DNA methylation reader complex that enhances gene transcription |
|
Relations |
BioSample |
SAMN08225546 |
SRA |
SRX3503898 |