|
| Status |
Public on Dec 01, 2018 |
| Title |
Brain_nonTreg_ischemia |
| Sample type |
RNA |
| |
|
| Source name |
non Tregs from ischemic brain at day14 after stroke onset
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Brain
cell: non Treg
|
| Treatment protocol |
For brain tissue, total RNA was isolated using QIAzol Lysis Reagent (QIAGEN) from the day 14 post-ischemic or sham brains of WT or DEREG mice (slice 3 on the ischemic side). For CD4+ T cells, total RNA was isolated using a TRIzolTM LS reagent (Invitrogen) from the CD4+ T cells in WT ischemic mice brain or in untreated mice spleen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For brain tissue, total RNA was isolated using QIAzol Lysis Reagent (QIAGEN) from the day 14 post-ischemic or sham brains of WT or DEREG mice (slice 3 on the ischemic side). For CD4+ T cells, total RNA was isolated using a TRIzolTM LS reagent (Invitrogen) from the CD4+ T cells in WT ischemic mice brain or in untreated mice spleen.
|
| Label |
Cy3
|
| Label protocol |
One-Color RNA Spike-In Kit, Low Input Quick Amp Labeling Kit (Agilent)
|
| |
|
| Hybridization protocol |
Expression Hybridization Kit (Agilent)
|
| Scan protocol |
DNA Microarray Scanner (2505C, Agilent), Gene Expression Wash Pack (Agilent)
|
| Data processing |
Feature Extraction ver 10.7.1.1 (Agielnt)
|
| |
|
| Submission date |
Jan 16, 2018 |
| Last update date |
Jan 07, 2019 |
| Contact name |
Minako Ito |
| E-mail(s) |
minakoito@bioreg.kyushu-u.ac.jp
|
| Organization name |
Kyushu University
|
| Street address |
3-1-1 Maidashi, Higashi-ku
|
| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE109259 |
Expression data in ischemic brain tissue and brain Tregs |
|
