|
| Status |
Public on Feb 13, 2018 |
| Title |
Patient Derived Xenograft LTL-484 |
| Sample type |
RNA |
| |
|
| Source name |
Prostate Tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: prostate cancer tumor
tumor foci: Patient Derived Xenograft
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted by using the mirVana RNA Isolation Kit (Ambion, Burlington, Canada). The quality and quantity of RNA were assessed with an Agilent 2100 Bioanalyzer (Caliper Technologies Corp., Canada)
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA was used as input for generation of Cyanine-3-labeled cRNA following Agilent’s One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling v6.0.
|
| |
|
| Hybridization protocol |
600ng labeled cRNA was hybridized on Agilent SurePrint G3 Human GE 8x60K Microarray (Design ID 028004)
|
| Scan protocol |
Slides were scanned with the Agilent DNA Microarray Scanner at a 3um scan resolution using the one colour and 20 bit tiff file settings
|
| Data processing |
Image files were processed with Agilent Feature Extraction 10.10 to produce spatially detrended and background subtracted gProcessedSignal intensities. Data were quantile normalized in GeneSpring 12.0.
|
| |
|
| Submission date |
Feb 13, 2018 |
| Last update date |
Feb 13, 2018 |
| Contact name |
Shawn Anderson |
| E-mail(s) |
Sanderson@prostatecentre.com
|
| Organization name |
Vancouver Prostate Centre
|
| Lab |
Laboratory for Advanced Genome Analysis
|
| Street address |
2660 Oak Street
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6H3Z6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE41192 |
High fidelity patient-derived xenografts for accelerating prostate cancer discovery and drug development (expression) |
| GSE41193 |
High fidelity patient-derived xenografts for accelerating prostate cancer discovery and drug development |
|
