|
| Status |
Public on Feb 09, 2021 |
| Title |
Cord Blood_TD069 |
| Sample type |
RNA |
| |
|
| Source name |
CBMC
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
batch: day4
estimated bcell: 0.363455698794661
estimated cd4t: 0.413890781165988
estimated cd8t: 0.112563259525123
estimated gran: 0
estimated mono: 0.129323037789338
estimated nk: -2.22415496137119e-19
estimated nrbc: 0.0498866175662087
gestational age: 37.71428571
birthweight sdscore: -1.686583438
|
| Treatment protocol |
Mononuclear cells were separated by gradient centrifugation using Ficoll-Hypaque from cord blood and postnatal peripheral blood samples within 24 h of the sample collection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
DNA extracted by Qiagen AllPrep kit.
|
| Label |
Cy3
|
| Label protocol |
Starting with 100 ng of the isolated RNA for each sample, double stranded cDNA and cyanine 3 labeled cRNA were synthesized using low input quick amp labeling kit, one-color and RNA spike-in kit, one-color (Agilent).
|
| |
|
| Hybridization protocol |
The resultant cRNAs were fragmented for 30 min at 60°C in dark. The fragmented cRNAs (600 ng) were hybridized on the microarray at 65°C for 17 h.
|
| Scan protocol |
After washing, slides were scanned with the Agilent microarray scanner G2505B.
|
| Data processing |
Feature extraction software (version 10.7.3.1) was used to calculate signal intensities from the scanned images.
|
| |
|
| Submission date |
Feb 20, 2018 |
| Last update date |
Feb 09, 2021 |
| Contact name |
Kohei Kashima |
| E-mail(s) |
KASHIMAK-PED@h.u-tokyo.ac.jp
|
| Phone |
+81-3-5800-8659
|
| Organization name |
The University of Tokyo Hospital
|
| Department |
Pediatrics
|
| Street address |
7-3-1 Hongo Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
1138655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21185 |
| Series (2) |
| GSE110822 |
Integrative analysis of DNA methylation and gene expression data among preterm and/or small for gestational age infants during perinatal period [expression] |
| GSE110829 |
Integrative analysis of DNA methylation and gene expression data among preterm and/or small for gestational age infants during perinatal period |
|
