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Sample GSM303502 Query DataSets for GSM303502
Status Public on Jul 30, 2008
Title 8 days cultures from wild-type mouse bone marrow progenitors - Rep 2
Sample type RNA
Source name 8 day cultured and sorted mature neutrophils
Organism Mus musculus
Characteristics Expression profile of mRNAs of 8 days cultured and sorted mature neutrophls from wild-type mouse bone marrow progenitors - Rep 2
Treatment protocol Cells were sorted for ckit-PE negative and Gr-1-APC positive population using FACSAria (BD Biosciences).
Growth protocol BM-progenitors were cultured in SILAC-IMDM with G-CSF and SCF for 6 days and subsequent culture without SCF for 42 hours.
Extracted molecule total RNA
Extraction protocol Tri Reagent (Ambion) followed by RNeasy Plus (Qiagen)
Label Biotin
Label protocol Affy Standard Labeling Protocol
Hybridization protocol Affy Standard Hyb Protocol
Scan protocol Affy Scan Protocol
Description mRNA expression profile comparison between wild-type cultured murine neutrophils versus miR-223 null cultured murine neutrophils during development of BM progenitors to mature neutrophils. This data shows expression profile of mRNAs from 8 days cultured and sorted mature neutrophils from wild-type mouse bone marrow.
Data processing GCOS and RMAExpress software
Submission date Jul 03, 2008
Last update date Aug 28, 2018
Contact name Whitehead Institute
Phone 617-324-0339
Organization name Whitehead Institute
Department Genome Technology Core
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
Platform ID GPL1261
Series (2)
GSE12003 4 days cultured progenitors and 8 days cultured mature neutrophils from WT vs miR-223 null neutrophils
GSE12075 The impact of microRNAs on protein output
Reanalyzed by GSE119085

Data table header descriptions
VALUE Signal Intensity (Log2)

Data table
1415670_AT 9.008118
1415671_AT 11.914763
1415672_AT 11.071206
1415673_AT 7.158685
1415674_A_AT 10.020844
1415675_AT 9.213312
1415676_A_AT 11.254409
1415677_AT 11.108843
1415678_AT 10.842527
1415679_AT 12.081774
1415680_AT 8.991669
1415681_AT 10.066113
1415682_AT 7.774070
1415683_AT 11.297064
1415684_AT 9.312492
1415685_AT 7.818942
1415686_AT 9.936309
1415687_A_AT 12.967032
1415688_AT 9.880333
1415689_S_AT 8.419950

Total number of rows: 45101

Table truncated, full table size 902 Kbytes.

Supplementary file Size Download File type/resource
GSM303502.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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