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Sample GSM304409 Query DataSets for GSM304409
Status Public on Oct 02, 2009
Title blood_responder_patient8
Sample type RNA
Source name Rheumatoid arthritis patient, whole blood, at 8:30-9:30 morning
Organism Homo sapiens
Characteristics disease state: responder
gender: female
tissue: whole blood
treatment stage: baseline (prior infliximab infusion)
Treatment protocol Paxgene tube conservation at -80ºC
Extracted molecule total RNA
Extraction protocol Whole blood RNA was extracted from whole blood preserved in Paxgene tubes using the PAXgene RNA Isolation kit (Qiagen)
Label Biotin
Label protocol Biotin labeled cRNA preparation for the Illumina platform was performed using the Ambion(r) Illumina RNA amplification kit (Ambion Europe, Huntington, Cambridgeshire, UK).
Hybridization protocol Biotin labeled cRNA (1.5ug) was hybridized to Sentrix(r) whole genome bead chips 6 x 2 (Illumina, San Diego, CA, USA)
Scan protocol Scanning was performed on the Illumina(r) BeadStation 500x.
Description Whole blood RNA, Infliximab responder
Data processing Log2 transformation of BeadStudio raw data, gene filtering of Beadchip version2 RefSeq valid probes, quantile normalization using R.
Submission date Jul 09, 2008
Last update date Mar 29, 2010
Contact name Antonio Julià
Phone +34 934029082
Organization name Vall d'Hebron Hospital Research Institute
Department Rheumatology Research Group
Lab Rheumatology Research Group
Street address c/ Baldiri Reixac nº15
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
Platform ID GPL2507
Series (1)
GSE12051 Microarray predictor of response to infliximab in rheumatoid arthritis (RA) patients

Data table header descriptions
VALUE Quantile normalized signal intensity

Data table
GI_10047089-S 6.57846062751213
GI_10047091-S 6.53252599908851
GI_10047093-S 6.73300060312058
GI_10047099-S 6.41728580624895
GI_10047103-S 8.44141610678198
GI_10047123-S 7.07526154486863
GI_10047133-I 6.46828300969387
GI_10092578-S 6.65702607519064
GI_10092585-S 6.87168562177214
GI_10092596-S 7.47114902495585
GI_10092600-S 7.40136992361908
GI_10092602-S 6.6163157420805
GI_10092611-A 7.8410910378902
GI_10092616-S 6.69452209389177
GI_10092618-S 9.42288861722012
GI_10092638-S 6.5892979565362
GI_10092658-S 6.64485886106863
GI_10092668-S 6.66668455766675
GI_10092672-S 7.31169080586854
GI_10190657-S 6.48617812475105

Total number of rows: 17454

Table truncated, full table size 521 Kbytes.

Supplementary file Size Download File type/resource
GSM304409.txt.gz 221.0 Kb (ftp)(http) TXT
Processed data included within Sample table

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