|
| Status |
Public on Dec 22, 2008 |
| Title |
Obese_surgery biopsy_subject 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
subcutaneous adipose tissue, obese surgery biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: Female, Biopsy Technique: surgical
|
| Treatment protocol |
tissue samples stored at -80°C until used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy total RNA Mini kit
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Linear Amplification kit
|
| |
|
| Channel 2 |
| Source name |
In-house adipose tissue reference pool
|
| Organism |
Homo sapiens |
| Characteristics |
subjects all undergoing plastic surgery
|
| Treatment protocol |
tissue samples stored at -80°C until used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy total RNA Mini kit
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input Linear Amplification kit
|
| |
|
| |
| Hybridization protocol |
Agilent Low RNA Input Linear Amplification kit
|
| Scan protocol |
microarrays scanned using an AXON scanner and data visualized using GenePix software
|
| Description |
Obese_surgery biopsy_subject 1
|
| Data processing |
Lowess normalized using Goulphar software, background signal not removed
|
| |
|
| Submission date |
Jul 10, 2008 |
| Last update date |
Dec 22, 2008 |
| Contact name |
David M Mutch |
| E-mail(s) |
dmmutch_sci@hotmail.com
|
| Phone |
+33-1-4234-6956
|
| Fax |
+33-1-4234-6993
|
| Organization name |
INSERM UMRS U872 (Eq 7) Nutriomique
|
| Department |
Nutrition and Metabolism
|
| Street address |
Centre de Recherche des Cordeliers (CRC), 15 rue de l'école de médecine
|
| City |
Paris |
| ZIP/Postal code |
75006 |
| Country |
France |
| |
|
| Platform ID |
GPL7038 |
| Series (1) |
| GSE12050 |
Subcutaneous adipose tissue from lean and obese subjects |
|
