|
Status |
Public on Dec 22, 2008 |
Title |
Obese_surgery biopsy_subject 2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
subcutaneous adipose tissue, obese surgery biopsy
|
Organism |
Homo sapiens |
Characteristics |
Gender: Female, Biopsy Technique: surgical
|
Treatment protocol |
tissue samples stored at -80°C until used
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy total RNA Mini kit
|
Label |
Cy5
|
Label protocol |
Agilent Low RNA Input Linear Amplification kit
|
|
|
Channel 2 |
Source name |
In-house adipose tissue reference pool
|
Organism |
Homo sapiens |
Characteristics |
subjects all undergoing plastic surgery
|
Treatment protocol |
tissue samples stored at -80°C until used
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy total RNA Mini kit
|
Label |
Cy3
|
Label protocol |
Agilent Low RNA Input Linear Amplification kit
|
|
|
|
Hybridization protocol |
Agilent Low RNA Input Linear Amplification kit
|
Scan protocol |
microarrays scanned using an AXON scanner and data visualized using GenePix software
|
Description |
Obese_surgery biopsy_subject 2
|
Data processing |
Lowess normalized using Goulphar software, background signal not removed
|
|
|
Submission date |
Jul 10, 2008 |
Last update date |
Dec 22, 2008 |
Contact name |
David M Mutch |
E-mail(s) |
dmmutch_sci@hotmail.com
|
Phone |
+33-1-4234-6956
|
Fax |
+33-1-4234-6993
|
Organization name |
INSERM UMRS U872 (Eq 7) Nutriomique
|
Department |
Nutrition and Metabolism
|
Street address |
Centre de Recherche des Cordeliers (CRC), 15 rue de l'école de médecine
|
City |
Paris |
ZIP/Postal code |
75006 |
Country |
France |
|
|
Platform ID |
GPL7038 |
Series (1) |
GSE12050 |
Subcutaneous adipose tissue from lean and obese subjects |
|