Human kidney mRNA (HuKi.basl) The mRNA was analyzed using Agilent's Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the publications : Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4):1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634. Briefly, the mRNA was reverse transcribed and the cDNA was digested with DpnII. The cDNA fragment starting at the 3' most DpnII site and ending with the polyA was cloned into a Megaclone vector library. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library HuKi.basl.ful01 Cell type Human kidney Age: 32-75 Sex: male/female number of donors: 10 Ethnicity: Caucasian RNA Source: Clontech cDNA library: Library DpnII restriction - (classic MPSS, cloning DpnII to poly A fragment) Sequence length 17 bp MPSS: runs HuKi.basl_ful01.1184T.a 11/22/2002 244808 11351 HuKi.basl_ful01.1184T.c 11/19/2002 184622 10650 HuKi.basl_ful01.1184T.d 11/19/2002 141295 9774 HuKi.basl_ful01.1184T.e 11/22/2002 227357 13674 HuKi.basl_ful01.1185F.b 11/20/2002 274063 10761 HuKi.basl_ful01.1185F.c 11/19/2002 202287 15883 HuKi.basl_ful01.1185F.d 11/19/2002 246552 17419 HuKi.basl_ful01.1185F.a 11/20/2002 298885 12749 HuKi.basl_ful01.1185F.e 11/25/2002 326833 20563 HuKi.basl_ful01.1185F.f 11/26/2002 352668 19631 Run group: Total Beads successfully sequenced - 2499370 Total Distinct Signatures - 30716 This data is also available on our signature genome browser at http://SGB.lynxgen.com and is described in details in 'C. Victor Jongeneel, Mauro Delorenzi, Christian Iseli, Daixing Zhou, Christian D. Haudenschild, Brian J. Stevenson, Robert L. Strausberg, Andrew J.G. Simpson, and Thomas J. Vasicek.' An atlas of human gene expression from massively parallel signature sequencing (MPSS). Genome Research VolXX 2004 Keywords = MPSS
