Sequencing from the 3' most DpnII site from mRNA using Lynx's MPSS technology. MPSS allows a comprehensive survey of all expressed transcripts in a cell or tissue, without apriori knowledge. This dataset was annotated using a combination of LocusLink, Unigene (build 171) and the latest human build as provided from UCSC (hg16). Annotation Classe Table: Virtual Signature Class mRNA Orientation Poly-Adenylation Features Position 0 Either - Repeat Warning Not applicable Not applicable 1 Forward Strand Poly-A Signal, Poly-A Tail 3' most 2 Forward Strand Poly-A Signal 3' most 3 Forward Strand Poly-A Tail 3' most 4 Forward Strand None 3' most 5 Forward Strand None Not 3' most 6 Forward Strand Internal Poly-A Not 3' most 11 Reverse Strand Poly-A Signal, Poly-A Tail 5' most 12 Reverse Strand Poly-A Signal 5' most 13 Reverse Strand Poly-A Tail 5' most 14 Reverse Strand None 5' most 15 Reverse Strand None Not 5' most 16 Reverse Strand Internal Poly-A Not 5' most 22 Unknown Poly-A Signal Last before signal 23 Unknown Poly-A Tail Last before tail 24 Unknown None Last in sequence 25 Unknown None Not last 26 Unknown Internal Poly-A Not 3' most 1000 Unknown- Derived from Genomic Sequence Not applicable Not applicable Keywords = MPSS, mRNA sequencing, tags
Signature - 17 bp sequence including DpnII site (GATC)
Significant
flag for signatures above 4 TPM (transcripts per million) in any of this dataset's sample. 's' means above 3 TPM, 'ns' below 4 TPM
Class
Annotation category according to Lynx's annotation system (see platform description).
Accession
LocusLink identifier or chromosome number if no extra annotation is available
Description
representative sequence accession number for the selected unigene/LocusLink hit or genomic coordinates if only genomic hit plus annotation if available
replication_global
Flag to signal if signature has been seen in more than one sequencing runs or MPSS flowcell.
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