Human pancreas (HuPn.basl) The mRNA was analyzed using Agilent's Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the publications : Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4):1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634. Briefly, the mRNA was reverse transcribed and the cDNA was digested with DpnII. The cDNA fragment starting at the 3' most DpnII site and ending with the polyA was cloned into a Megaclone vector library. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library HuPn.basl.ful01 Cell type Human pancreas Age: 25-59 Sex: male/female number of donors: 20 Ethnicity: Caucasian RNA Source: Clontech cDNA library: Library DpnII restriction - (classic MPSS, cloning DpnII to poly A fragment) Sequence length 17 bp MPSS: runs HuPn.basl_ful01.1224T.a 11/23/2002 304739 6577 HuPn.basl_ful01.3055T.a 12/5/2002 388063 4926 HuPn.basl_ful01.3055T.b 12/8/2002 411868 5053 HuPn.basl_ful01.3055T.c 12/7/2002 396092 4601 HuPn.basl_ful01.3424T.a 12/23/2002 701976 13644 HuPn.basl_ful01.3424T.b 12/25/2002 594078 12450 HuPn.basl_ful01.1225F.b 11/26/2002 303632 5567 HuPn.basl_ful01.3055F.a 12/8/2002 299961 3350 HuPn.basl_ful01.3055F.b 12/7/2002 285890 3031 HuPn.basl_ful01.3055F.c 12/7/2002 355559 3327 HuPn.basl_ful01.3055F.d 12/7/2002 336441 3220 HuPn.basl_ful01.3424F.b 12/23/2002 518866 10272 HuPn.basl_ful01.3424F.a 12/23/2002 426952 9089 Run group: Total Beads successfully sequenced - 5324117 Total Distinct Signatures - 16238 This data is also available on our signature genome browser at http://SGB.lynxgen.com and is described in details in 'C. Victor Jongeneel, Mauro Delorenzi, Christian Iseli, Daixing Zhou, Christian D. Haudenschild, Brian J. Stevenson, Robert L. Strausberg, Andrew J.G. Simpson, and Thomas J. Vasicek.' An atlas of human gene expression from massively parallel signature sequencing (MPSS). Genome Research VolXX 2004 Keywords = MPSS
