Human placenta mRNA (HuPl.basl) The mRNA was analyzed using Agilent's Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the publications : Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4):1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634. Briefly, the mRNA was reverse transcribed and the cDNA was digested with DpnII. The cDNA fragment starting at the 3' most DpnII site and ending with the polyA was cloned into a Megaclone vector library. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library HuPl.basl.ful01 Cell type Human placenta Age: 23-35 Sex: female number of donors: 4 Ethnicity: Caucasian RNA Source: Clontech cDNA library: Library DpnII restriction - (classic MPSS, cloning DpnII to poly A fragment) Sequence length 17 bp MPSS: runs HuPl.basl_ful01.1134T.d 11/19/2002 353869 12906 HuPl.basl_ful01.1134T.c 11/19/2002 328537 10442 HuPl.basl_ful01.1134T.e 11/25/2002 314346 15431 HuPl.basl_ful01.1135F.a 11/22/2002 376200 13030 HuPl.basl_ful01.1135F.c 11/19/2002 405457 14450 HuPl.basl_ful01.1135F.d 11/20/2002 439634 13763 Run group: Total Beads successfully sequenced - 2218043 Total Distinct Signatures - 23101 This data is also available on our signature genome browser at http://SGB.lynxgen.com and is described in details in 'C. Victor Jongeneel, Mauro Delorenzi, Christian Iseli, Daixing Zhou, Christian D. Haudenschild, Brian J. Stevenson, Robert L. Strausberg, Andrew J.G. Simpson, and Thomas J. Vasicek.' An atlas of human gene expression from massively parallel signature sequencing (MPSS). Genome Research VolXX 2004 Keywords = MPSS
