Human Small Intestine (HuSI.basl) The mRNA was analyzed using Agilent's Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the publications : Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4):1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634. Briefly, the mRNA was reverse transcribed and the cDNA was digested with DpnII. The cDNA fragment starting at the 3' most DpnII site and ending with the polyA was cloned into a Megaclone vector library. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library HuSI.basl.ful01 Cell type Human Small Intestine Age: 15-60 Sex: male/female number of donors: 11 Ethnicity: Caucasian RNA Source: Clontech cDNA library: Library DpnII restriction - (classic MPSS, cloning DpnII to poly A fragment) Sequence length 17 bp MPSS: runs HuSI.basl_ful01.1242T.c 12/3/2002 332357 14482 HuSI.basl_ful01.1242T.d 12/3/2002 350397 15162 HuSI.basl_ful01.1242T.a 12/3/2002 237411 12068 HuSI.basl_ful01.1242T.b 12/3/2002 232174 11837 HuSI.basl_ful01.3354T.b 12/23/2002 523214 27448 HuSI.basl_ful01.3354T.a 12/26/2002 532194 27633 HuSI.basl_ful01.3354T.c 12/24/2002 530231 27873 HuSI.basl_ful01.3354F.b 12/23/2002 518788 26371 HuSI.basl_ful01.3354F.a 12/23/2002 607788 28285 HuSI.basl_ful01.3354F.c 12/24/2002 382954 23438 Run group: Total Beads successfully sequenced - 4247508 Total Distinct Signatures - 40072 This data is also available on our signature genome browser at http://SGB.lynxgen.com and is described in details in 'C. Victor Jongeneel, Mauro Delorenzi, Christian Iseli, Daixing Zhou, Christian D. Haudenschild, Brian J. Stevenson, Robert L. Strausberg, Andrew J.G. Simpson, and Thomas J. Vasicek.' An atlas of human gene expression from massively parallel signature sequencing (MPSS). Genome Research VolXX 2004 Keywords = MPSS
