Human Spleen (HuSp.basl) The mRNA was analyzed using Agilent's Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the publications : Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4):1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634. Briefly, the mRNA was reverse transcribed and the cDNA was digested with DpnII. The cDNA fragment starting at the 3' most DpnII site and ending with the polyA was cloned into a Megaclone vector library. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library HuSp.basl.ful01 Cell type Human Spleen Age: 24-61 Sex: male/female number of donors: 6 Ethnicity: Caucasian RNA Source: Clontech cDNA library: Library DpnII restriction - (classic MPSS, cloning DpnII to poly A fragment) Sequence length 17 bp MPSS: runs HuSp.basl_ful01.3332T.a 12/19/2002 660778 34936 HuSp.basl_ful01.3332T.b 12/19/2002 674214 35652 HuSp.basl_ful01.3332T.c 12/19/2002 351659 26438 HuSp.basl_ful01.3332F.a 12/20/2002 738376 36241 HuSp.basl_ful01.3332F.b 12/20/2002 722998 34163 Run group: Total Beads successfully sequenced - 3148025 Total Distinct Signatures - 52274 This data is also available on our signature genome browser at http://SGB.lynxgen.com and is described in details in 'C. Victor Jongeneel, Mauro Delorenzi, Christian Iseli, Daixing Zhou, Christian D. Haudenschild, Brian J. Stevenson, Robert L. Strausberg, Andrew J.G. Simpson, and Thomas J. Vasicek.' An atlas of human gene expression from massively parallel signature sequencing (MPSS). Genome Research VolXX 2004 Keywords = MPSS
