Human Testis (HuTe.basl) The mRNA was analyzed using Agilent's Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the publications : Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4):1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634. Briefly, the mRNA was reverse transcribed and the cDNA was digested with DpnII. The cDNA fragment starting at the 3' most DpnII site and ending with the polyA was cloned into a Megaclone vector library. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library HuTe.basl.ful01 Cell type Human Testis Age: 28-64 Sex: male number of donors: 25 Ethnicity: Caucasian RNA Source: Clontech cDNA library: Library DpnII restriction - (classic MPSS, cloning DpnII to poly A fragment) Sequence length 17 bp MPSS: runs HuTe.basl_ful01.1263T.a 11/20/2002 251658 24925 HuTe.basl_ful01.1263T.b 11/25/2002 220925 18237 HuTe.basl_ful01.3052T.b 12/8/2002 386249 38437 HuTe.basl_ful01.3052T.a 12/9/2002 426304 40974 HuTe.basl_ful01.3052T.c 12/10/2002 416732 38630 HuTe.basl_ful01.3052F.a 12/9/2002 431688 39852 HuTe.basl_ful01.3052F.b 12/9/2002 449586 41210 HuTe.basl_ful01.3052F.c 12/10/2002 425433 36687 HuTe.basl_ful01.3052F.d 12/14/2002 342235 37069 Run group: Total Beads successfully sequenced - 3350810 Total Distinct Signatures - 65706 This data is also available on our signature genome browser at http://SGB.lynxgen.com and is described in details in 'C. Victor Jongeneel, Mauro Delorenzi, Christian Iseli, Daixing Zhou, Christian D. Haudenschild, Brian J. Stevenson, Robert L. Strausberg, Andrew J.G. Simpson, and Thomas J. Vasicek.' An atlas of human gene expression from massively parallel signature sequencing (MPSS). Genome Research VolXX 2004 Keywords = MPSS
