Human thymus mRNA (HuTm.basl) The mRNA was analyzed using Agilent's Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the publications : Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4):1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634. Briefly, the mRNA was reverse transcribed and the cDNA was digested with DpnII. The cDNA fragment starting at the 3' most DpnII site and ending with the polyA was cloned into a Megaclone vector library. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library HuTm.basl.ful01 Cell type Human thymus Age: 18-32 Sex: male/female number of donors: 9 Ethnicity: Caucasian RNA Source: Clontech cDNA library: Library DpnII restriction - (classic MPSS, cloning DpnII to poly A fragment) Sequence length 17 bp MPSS: runs HuTm.basl_ful01.3322T.a 12/19/2002 636266 33167 HuTm.basl_ful01.3322T.b 12/19/2002 837729 37574 HuTm.basl_ful01.3322T.c 12/19/2002 835881 35247 HuTm.basl_ful01.3322T.d 12/20/2002 338873 22776 HuTm.basl_ful01.3101-RF.b 12/25/2002 510883 30853 HuTm.basl_ful01.3101-TF.b 12/25/2002 435461 29551 HuTm.basl_ful01.3101-RF.c 12/14/2002 497400 30744 HuTm.basl_ful01.3101-TF.c 12/14/2002 477622 30244 HuTm.basl_ful01.3101-RF.a 12/15/2002 461361 29108 HuTm.basl_ful01.3101-TF.a 12/15/2002 493879 29905 Run group: Total Beads successfully sequenced - 5525355 Total Distinct Signatures - 54492 This data is also available on our signature genome browser at http://SGB.lynxgen.com and is described in details in 'C. Victor Jongeneel, Mauro Delorenzi, Christian Iseli, Daixing Zhou, Christian D. Haudenschild, Brian J. Stevenson, Robert L. Strausberg, Andrew J.G. Simpson, and Thomas J. Vasicek.' An atlas of human gene expression from massively parallel signature sequencing (MPSS). Genome Research VolXX 2004 Keywords = MPSS
