Human trachea mRNA (HuTr.basl) The mRNA was analyzed using Agilent's Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the publications : Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4):1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634. Briefly, the mRNA was reverse transcribed and the cDNA was digested with DpnII. The cDNA fragment starting at the 3' most DpnII site and ending with the polyA was cloned into a Megaclone vector library. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library HuTr.basl.ful01 Cell type Human trachea Age: 17-70 Sex: male/female number of donors: 84 Ethnicity: Caucasian RNA Source: Clontech cDNA library: Library DpnII restriction - (classic MPSS, cloning DpnII to poly A fragment) Sequence length 17 bp MPSS: runs HuTr.basl_ful01.3104-7T.a 12/9/2002 556300 25373 HuTr.basl_ful01.3104-6T.b 12/9/2002 303896 17146 HuTr.basl_ful01.3104-7T.b 12/8/2002 501798 25257 HuTr.basl_ful01.3104-6T.c 12/14/2002 325766 18594 HuTr.basl_ful01.3104-7T.c 12/14/2002 502880 24542 HuTr.basl_ful01.3104-6T.d 12/15/2002 370648 19950 HuTr.basl_ful01.3104-7T.d 12/15/2002 303833 20064 HuTr.basl_ful01.3111T.a 12/15/2002 357697 19069 HuTr.basl_ful01.3104-6F.a 12/8/2002 294602 18174 HuTr.basl_ful01.3104-7F.a 12/8/2002 401488 22404 HuTr.basl_ful01.3104-7F.c 12/14/2002 543485 25612 HuTr.basl_ful01.3104-6F.b 12/14/2002 342746 19366 HuTr.basl_ful01.3104-7F.b 12/14/2002 376280 21284 HuTr.basl_ful01.3111F.a 12/15/2002 345430 18750 HuTr.basl_ful01.3111F.b 12/15/2002 474403 22593 Run group: Total Beads successfully sequenced - 6001252 Total Distinct Signatures - 38615 This data is also available on our signature genome browser at http://SGB.lynxgen.com and is described in details in 'C. Victor Jongeneel, Mauro Delorenzi, Christian Iseli, Daixing Zhou, Christian D. Haudenschild, Brian J. Stevenson, Robert L. Strausberg, Andrew J.G. Simpson, and Thomas J. Vasicek.' An atlas of human gene expression from massively parallel signature sequencing (MPSS). Genome Research VolXX 2004 Keywords = MPSS
