|
Status |
Public on Jun 05, 2012 |
Title |
slide 20-lumiestrone experiment |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
vehicle control
|
Organism |
Carassius auratus |
Characteristics |
Sexually inactive male adult goldfish; liver tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Plus kit (Invitrogen)
|
Label |
Cy5
|
Label protocol |
Array 900MPX™ Expression Array Detection Kit (Genisphere; see http://www.genisphere.com/array_detection_protocols.html)
|
|
|
Channel 2 |
Source name |
luminestrone-injected (0.5 ug/g)
|
Organism |
Carassius auratus |
Characteristics |
Sexually inactive male adult goldfish; liver tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Plus kit (Invitrogen)
|
Label |
Cy3
|
Label protocol |
Array 900MPX™ Expression Array Detection Kit (Genisphere; see http://www.genisphere.com/array_detection_protocols.html)
|
|
|
|
Hybridization protocol |
Array 900MPX™ Expression Array Detection Kit (Genisphere; see http://www.genisphere.com/array_detection_protocols.html)
|
Scan protocol |
Microarrays were scanned at full speed 10 micron resolution using the ScanArray 5000 XL system (Packard Biosciences/PerkinElmer) using both red and blue lasers. Images were obtained with ScanArray Express software using automatic calibration sensitivity varying PMT gain (PMT starting at 65% for Cy5 and 70% for Cy3) with fixed laser power at 80% and the target intensity set for 90%.
|
Description |
Sexually inactive male goldfish were injected i.p. with lumiestrone (0.5 µg/g) or control vehicle (in charcoal-stripped peanut oil; 5 µL/g). Animals were anaesthetized in MS222, and livers were harvested for RNA extraction, cDNA synthesis and microarray analysis. Slide #20
|
Data processing |
Microarray images were analyzed using QuantArray (Packard Biosciences/PerkinElmer) and raw signal intensity values were obtained for duplicate spots of genes. Normalization was performed using LOWESS and scale methods using the Alien Spot Report Genes 1-10 (Stratagene).
|
|
|
Submission date |
Jul 25, 2008 |
Last update date |
Jun 05, 2012 |
Contact name |
Jason T. Popesku |
E-mail(s) |
jpope078@uottawa.ca
|
Organization name |
University of Ottawa
|
Department |
Biology
|
Lab |
Trudeau
|
Street address |
20 Marie Curie
|
City |
Ottawa |
State/province |
ON |
ZIP/Postal code |
K1N 6N5 |
Country |
Canada |
|
|
Platform ID |
GPL7056 |
Series (1) |
GSE12252 |
AURATUS goldfish environmental genomics projects: lumiestrone in the liver |
|