|
| Status |
Public on Jun 05, 2012 |
| Title |
slide 20-lumiestrone experiment |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
vehicle control
|
| Organism |
Carassius auratus |
| Characteristics |
Sexually inactive male adult goldfish; liver tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Plus kit (Invitrogen)
|
| Label |
Cy5
|
| Label protocol |
Array 900MPX™ Expression Array Detection Kit (Genisphere; see http://www.genisphere.com/array_detection_protocols.html)
|
| |
|
| Channel 2 |
| Source name |
luminestrone-injected (0.5 ug/g)
|
| Organism |
Carassius auratus |
| Characteristics |
Sexually inactive male adult goldfish; liver tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Plus kit (Invitrogen)
|
| Label |
Cy3
|
| Label protocol |
Array 900MPX™ Expression Array Detection Kit (Genisphere; see http://www.genisphere.com/array_detection_protocols.html)
|
| |
|
| |
| Hybridization protocol |
Array 900MPX™ Expression Array Detection Kit (Genisphere; see http://www.genisphere.com/array_detection_protocols.html)
|
| Scan protocol |
Microarrays were scanned at full speed 10 micron resolution using the ScanArray 5000 XL system (Packard Biosciences/PerkinElmer) using both red and blue lasers. Images were obtained with ScanArray Express software using automatic calibration sensitivity varying PMT gain (PMT starting at 65% for Cy5 and 70% for Cy3) with fixed laser power at 80% and the target intensity set for 90%.
|
| Description |
Sexually inactive male goldfish were injected i.p. with lumiestrone (0.5 µg/g) or control vehicle (in charcoal-stripped peanut oil; 5 µL/g). Animals were anaesthetized in MS222, and livers were harvested for RNA extraction, cDNA synthesis and microarray analysis.
Slide #20
|
| Data processing |
Microarray images were analyzed using QuantArray (Packard Biosciences/PerkinElmer) and raw signal intensity values were obtained for duplicate spots of genes. Normalization was performed using LOWESS and scale methods using the Alien Spot Report Genes 1-10 (Stratagene).
|
| |
|
| Submission date |
Jul 25, 2008 |
| Last update date |
Jun 05, 2012 |
| Contact name |
Jason T. Popesku |
| E-mail(s) |
jpope078@uottawa.ca
|
| Organization name |
University of Ottawa
|
| Department |
Biology
|
| Lab |
Trudeau
|
| Street address |
20 Marie Curie
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1N 6N5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL7056 |
| Series (1) |
| GSE12252 |
AURATUS goldfish environmental genomics projects: lumiestrone in the liver |
|
