|
| Status |
Public on Aug 16, 2018 |
| Title |
Glucagon 100nM_6h_Experiment 2 |
| Sample type |
RNA |
| |
|
| Source name |
Glucagon 100nM_6h
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6J
Sex: male
cell type: Mouse primary hepatocytes (MPH)
treated with: Glucagon 100nM for 6hrs
|
| Treatment protocol |
Glucagon 100nM +/-GW4064 2µM during 6h
|
| Growth protocol |
DMEM 1g/l de glucose w/o glutamin + 0,1% BSA + 1% glutamin + 100 nM dexametason
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extract-All extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated ssDNA were prepared according to the standard Affymetrix protocol from 300ng total RNA (GeneChip® WT PLUS Reagent Kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5,5µg of ssDNA were hybridized for 16 hr at 45C on GeneChip® Mouse Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner GCS3000.
|
| Description |
28-29_(MoGene-2_0-st).CEL
|
| Data processing |
The data were analyzed with Genespring 14,3 software using global scaling as normalization method.
|
| |
|
| Submission date |
Apr 23, 2018 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Lefebvre Philippe |
| Organization name |
Inserm
|
| Lab |
JK
|
| Street address |
Bld Pr Jules Leclerc
|
| City |
Lille |
| ZIP/Postal code |
59000 |
| Country |
France |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE113526 |
The nuclear Bile Acid Receptor FXR is a PKA- and FOXA2- sensitive Activator of Fasting Hepatic Gluconeogenesis [glucacon/GW4064] |
| GSE113575 |
The nuclear Bile Acid Receptor FXR is a PKA- and FOXA2- sensitive Activator of Fasting Hepatic Gluconeogenesis |
|
