|
Status |
Public on Aug 16, 2018 |
Title |
GW4064 2µM_6h_Experiment 2 |
Sample type |
RNA |
|
|
Source name |
GW4064 2µM_6h
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl6J Sex: male cell type: Mouse primary hepatocytes (MPH) treated with: GW4064 2µM for 6hrs
|
Treatment protocol |
Glucagon 100nM +/-GW4064 2µM during 6h
|
Growth protocol |
DMEM 1g/l de glucose w/o glutamin + 0,1% BSA + 1% glutamin + 100 nM dexametason
|
Extracted molecule |
total RNA |
Extraction protocol |
Extract-All extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated ssDNA were prepared according to the standard Affymetrix protocol from 300ng total RNA (GeneChip® WT PLUS Reagent Kit, Affymetrix).
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Hybridization protocol |
Following fragmentation, 5,5µg of ssDNA were hybridized for 16 hr at 45C on GeneChip® Mouse Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using GeneChip Scanner GCS3000.
|
Description |
32_33bis_(MoGene-2_0-st).CEL
|
Data processing |
The data were analyzed with Genespring 14,3 software using global scaling as normalization method.
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|
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Submission date |
Apr 23, 2018 |
Last update date |
Aug 16, 2018 |
Contact name |
Lefebvre Philippe |
Organization name |
Inserm
|
Lab |
JK
|
Street address |
Bld Pr Jules Leclerc
|
City |
Lille |
ZIP/Postal code |
59000 |
Country |
France |
|
|
Platform ID |
GPL16570 |
Series (2) |
GSE113526 |
The nuclear Bile Acid Receptor FXR is a PKA- and FOXA2- sensitive Activator of Fasting Hepatic Gluconeogenesis [glucacon/GW4064] |
GSE113575 |
The nuclear Bile Acid Receptor FXR is a PKA- and FOXA2- sensitive Activator of Fasting Hepatic Gluconeogenesis |
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