|
| Status |
Public on May 06, 2019 |
| Title |
leaf_crTMV inoculated_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
leaf
|
| Organism |
Nicotiana benthamiana |
| Characteristics |
infection: crTMV
|
| Treatment protocol |
N. benthamiana was infected with Cymbidium ring spot virus (CymRSV), turnip crinkle virus (TCV), crucifer infecting tobacco mosaic virus (crTMV). Host plants were used for infections using a sap inoculation with in vitro transcripts of the viruses (crTMV) or with purified virions (CymRSV, TCV).
|
| Growth protocol |
Plants were grown in soil under 16 h light /8 h dark regime at 21°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA from control leaves and virus infected, systemically symptomatic leaves was extracted with phenol-chloroform methods (White and Kaper 1989)
|
| Label |
Cy3
|
| Label protocol |
Low Input QuickAmp 6.5
|
| |
|
| Hybridization protocol |
One-Color Microarray-Based Gene Expression AnalysisLow Input Quick Amp Labeling
|
| Scan protocol |
Agilent scanner, FE SW 9.5 Extended Dynamic Range (with laser intensity at 100% and 10%; with 5 micron resolutino)
|
| Data processing |
Feature Extraction Software 10.01., default one color, high density, protocol (GE1-v5_95_Feb07)
|
| |
|
| Submission date |
Apr 27, 2018 |
| Last update date |
May 06, 2019 |
| Contact name |
Levente Kontra |
| E-mail(s) |
Kontra.levente@gmail.com
|
| Organization name |
HUNREN
|
| Department |
institute of experimental medicine
|
| Lab |
Translational Behavioural Neuroscience Research Group
|
| Street address |
Szigony st 43.
|
| City |
Budapest |
| ZIP/Postal code |
H-1083 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL21056 |
| Series (2) |
| GSE113772 |
Differential gene expression and physiological changes during acute or persistent plant virus interactions may contribute to viral symptom differences (microarray) |
| GSE113774 |
Differential gene expression and physiological changes during acute or persistent plant virus interactions may contribute to viral symptom differences |
|
