|
| Status |
Public on Apr 01, 2009 |
| Title |
Chondrosarcoma_case_17 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Case 17
|
| Organism |
Homo sapiens |
| Characteristics |
Genomic DNA from fresh frozen chondrosarcoma biopsy
|
| Biomaterial provider |
Dept of Clinical Genetics, Lund University Hospital, Sweden
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNeasy Tissue Kit (Qiagen, Valencia, CA, USA)
|
| Label |
Cy3
|
| Label protocol |
BioPrime array CGH labeling module (Invitrogen, Carlsbad, CA, USA)
|
| |
|
| Channel 2 |
| Source name |
Promega reference male DNA (Promega, Madison, WI, USA)
|
| Organism |
Homo sapiens |
| Characteristics |
Pooled lymphoblastoid genomic DNA from ten male individuals
|
| Biomaterial provider |
Promega, Madison, WI, USA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
none
|
| Label |
Cy5
|
| Label protocol |
BioPrime array CGH labeling module (Invitrogen, Carlsbad, CA, USA)
|
| |
|
| |
| Hybridization protocol |
Arrays were hybridized and washed as described in Heidenblad et al. (Oncogene 2006;25:7106-16)
|
| Scan protocol |
Arrays were scanned in Agilent Microarray scanner
|
| Description |
32k BAC array CGH data on chondrosarcoma
|
| Data processing |
Primary data were collected using the GenePix Pro 4.0 software, and the quantified data matrix was deposited into the web-based database BioArray Software Environment (BASE), where filtering and lowess normalization were performed.
|
| |
|
| Submission date |
Aug 13, 2008 |
| Last update date |
Feb 25, 2009 |
| Contact name |
Karolin H Nord |
| Organization name |
Lund University
|
| Department |
Dept of Clinical Genetics
|
| Street address |
BMC C13
|
| City |
Lund |
| ZIP/Postal code |
SE-22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL4723 |
| Series (2) |
| GSE12439 |
Genomic profiling of chondrosarcoma: chromosomal patterns in central and peripheral tumors (BAC/CGH) |
| GSE12532 |
Genomic profiling of chondrosarcoma: chromosomal patterns in central and peripheral tumors |
|
