|
| Status |
Public on Apr 23, 2009 |
| Title |
Brain-CTRL18-ArrayG-rep1 |
| Sample type |
genomic |
| |
|
| Source name |
Control Brain (Brodmann area 46)
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: male
Age: 74 y
Phenotype: normal
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Phenol chloroform extraction of human brain tissue. Genpathway Inc performed the following: DNA (12 ug) was precleared with protein G agarose beads (Invitrogen). Methylated DNA was detected using a sheep polyclonal antibody against 5-methyl-cytosine. After incubation at 4C overnight, protein G agarose beads were used to isolate the immune complexes. Complexes were washed and eluted from the beads with SDS buffer. IP’ed DNA was purified by phenol/chloroform extraction and ethanol precipitation.
|
| Label |
biotin
|
| Label protocol |
Amplified DNAs were fragmented and labeled using the Affymetrix DNA Terminal Labeling Kit (Genpathway Inc.)
|
| |
|
| Hybridization protocol |
Hybridization to Affymetrix GeneChip Human Tiling 2.0R arrays at 45 degrees overnight (Genpathway Inc.)
|
| Scan protocol |
Arrays were washed and scanned (Genpathway Inc.)
|
| Description |
Affymetrix dsDNA terminal label (Genpathway Inc.)
MeDIP data from normal control brain
|
| Data processing |
Data processed using Affymetrix TAS software and Genpathway IP analysis software (Genpathway Inc.)
|
| |
|
| Submission date |
Aug 22, 2008 |
| Last update date |
Apr 23, 2009 |
| Contact name |
Roger Pamphlett |
| Organization name |
University of Sydney
|
| Street address |
Blackburn Bld
|
| City |
University of Sydney |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL4916 |
| Series (1) |
| GSE12525 |
5-methylcytosine ChIP-on-Chip (MeDIP) of sporadic amyotrophic lateral sclerosis and control human brains |
|
