|
| Status |
Public on Sep 03, 2008 |
| Title |
Xenopus laevis Brain, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Xenopus laevis, Adult male hindbrain (dorsal tegmental area of the medulla to the end of n.IX-X)
|
| Organism |
Xenopus laevis |
| Characteristics |
Adult male Xenopus laevis
|
| Treatment protocol |
Animal was immersed in MS222 and tissue was immediately extracted
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol® Reagent (Invitrogen Life Technologies) according to the manufacturer’s protocol and purified with RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Two micrograms of total RNA was used to prepare biotin labeled cRNA probes following the manufacturer’s (Affymetrix) protocol.
|
| |
|
| Hybridization protocol |
Hybridization to Affymetrix Xenopus laevis Genome expression array followed the manufacturer's (Affymetrix) protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
1 of 3 biological replicates of gene expression data from adult brain.
|
| Data processing |
Data were RMA normalized using RMA Express and a probemask (based on perfect match probes for X.laevis and X.borealis).
Normalization of 3 biological replicates were performed with 3 biological replicates of X.laevis testis
|
| |
|
| Submission date |
Aug 29, 2008 |
| Last update date |
Dec 03, 2008 |
| Contact name |
Frederic Chain |
| E-mail(s) |
chainfj@mcmaster.ca
|
| Organization name |
McMaster University
|
| Department |
Biology
|
| Lab |
LSB 324
|
| Street address |
1280 Main Street West
|
| City |
Hamilton |
| State/province |
Ontario |
| ZIP/Postal code |
L8S4K1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1318 |
| Series (1) |
| GSE12625 |
Expression data for a study on cross-species hybridization on single-species microarrays |
|
