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Sample GSM316736 Query DataSets for GSM316736
Status Public on Sep 03, 2008
Title Xenopus laevis Brain, biological replicate 2
Sample type RNA
Source name Xenopus laevis, Adult male hindbrain (dorsal tegmental area of the medulla to the end of n.IX-X)
Organism Xenopus laevis
Characteristics Adult male Xenopus laevis
Treatment protocol Animal was immersed in MS222 and tissue was immediately extracted
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol® Reagent (Invitrogen Life Technologies) according to the manufacturer’s protocol and purified with RNeasy Mini Kit (Qiagen).
Label biotin
Label protocol Two micrograms of total RNA was used to prepare biotin labeled cRNA probes following the manufacturer’s (Affymetrix) protocol.
Hybridization protocol Hybridization to Affymetrix Xenopus laevis Genome expression array followed the manufacturer's (Affymetrix) protocol.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description 1 of 3 biological replicates of gene expression data from adult brain.
Data processing Data were RMA normalized using RMA Express and a probemask (based on perfect match probes for X.laevis and X.borealis).
Normalization of 3 biological replicates were performed with 3 biological replicates of X.laevis testis
Submission date Aug 29, 2008
Last update date Dec 03, 2008
Contact name Frederic Chain
Organization name McMaster University
Department Biology
Lab LSB 324
Street address 1280 Main Street West
City Hamilton
State/province Ontario
ZIP/Postal code L8S4K1
Country Canada
Platform ID GPL1318
Series (1)
GSE12625 Expression data for a study on cross-species hybridization on single-species microarrays

Data table header descriptions
VALUE RMA normalized (log2) using RMA express using "XB+XL perfect match probemask"

Data table
Xl.1.1.S1_s_at 12.27039082
Xl.10012.1.A1_at 3.828855158
Xl.10032.1.A1_s_at 8.25128246
Xl.10043.1.A1_at 6.433248836
Xl.10043.2.S1_at 4.371447276
Xl.10054.1.S1_at 8.053811129
Xl.10087.1.A1_at 10.69517925
Xl.10104.1.A1_at 7.354419833
Xl.10118.1.S1_at 5.998635715
Xl.1013.1.S1_at 4.217866835
Xl.10142.1.S1_at 7.07040617
Xl.1015.1.S1_at 6.830190093
Xl.10170.1.A1_at 7.793601671
Xl.10191.1.S1_at 7.388766663
Xl.10208.1.S1_at 7.170828026
Xl.10222.1.A1_at 5.405153209
Xl.10227.1.A1_at 8.192549167
Xl.10230.1.A1_at 7.304798922
Xl.10251.1.A1_at 9.001765598
Xl.10287.1.S1_at 10.2268059

Total number of rows: 2143

Table truncated, full table size 59 Kbytes.

Supplementary file Size Download File type/resource
GSM316736.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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