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Status |
Public on Sep 16, 2008 |
Title |
Parental Yeast alpha-type Cell Replicate 1 |
Sample type |
genomic |
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Channel 1 |
Source name |
S. bayanus alpha-type cells
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Organism |
Saccharomyces bayanus |
Characteristics |
strain: S. bayanus#180
|
Growth protocol |
Yeast cells were grown in YPD medium, at 30 degree
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Yeast genomic DNA was extracted using QIAGEN Genomic-tip 100/G kit
|
Label |
Cy5
|
Label protocol |
fragmentation protocol:4.5 µg total genomic DNA were digested by 1 µl Dnase I (0.05 U) at 37 degree for 5 minutes and at 100 degree, 15 minutes for enzyme inactivation. The label protocol was adapted from Microarray Core Facility, Insititute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html)
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Channel 2 |
Source name |
S. cerevisiae alpha-type cells
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: W303
|
Growth protocol |
Yeast cells were grown in YPD medium, at 30 degree
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Yeast genomic DNA was extracted using QIAGEN Genomic-tip 100/G kit
|
Label |
Cy3
|
Label protocol |
fragmentation protocol:4.5 µg total genomic DNA were digested by 1 µl Dnase I (0.05 U) at 37 degree for 5 minutes and at 100 degree, 15 minutes for enzyme inactivation. The label protocol was adapted from Microarray Core Facility, Insititute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html)
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Hybridization protocol |
The hybridization protocol was adapted from Microarray Core Facility, Insititute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html)
|
Scan protocol |
Arrays were scanned for F635(cy5) and F532(Cy3) fluoresence intensities using AXON 4000B ( Molecular Device , sunnyvale , CA )
|
Description |
The S. cerevisiae hybrid karyotypes were analyzed by array-CGH to identify small regions of duplication or homeologous chromosomal exchange occurring during the strain construction.
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Data processing |
Subtracted background. Dividing by ch2 (control) was used to transform data (Agilent GeneSpring 7.3.1 software)
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Submission date |
Sep 15, 2008 |
Last update date |
Sep 15, 2008 |
Contact name |
Jun-Yi Leu |
E-mail(s) |
jleu@imb.sinica.edu.tw
|
Organization name |
Academia Sinica
|
Department |
Insititute of Molecular Biolody
|
Street address |
128 Academia Road, Section 2, Nankang
|
City |
Taipei |
ZIP/Postal code |
115 |
Country |
Taiwan |
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|
Platform ID |
GPL7305 |
Series (2) |
GSE12776 |
Karyotype analysis of S. cerevisiae chromosome replacement lines - alpha-type cells |
GSE12785 |
Karyotype analysis of S. cerevisiae chromosome replacement lines |
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