Cells were grown in M63 glucose (0.2%) minimal media at 37oC at 200 rpm. Overnight cultures were inoculated with single, independent colonies in triplicate, incubated aerobically overnight at 37oC, and sub-cultured to a starting OD600 of 0.0001. Cultures were harvested at an OD600 = 0.3 after being maintained in exponential phase for at least eight generations.
Extracted molecule
total RNA
Extraction protocol
Acidic Hot Phenol
Label
biotin
Label protocol
Affymetrix protocol
Hybridization protocol
Affymetrix protocol
Scan protocol
Affymetrix protocol
Description
This study is to identify the RpoS regulon in MG1655 grown in M63 glucose (0.2%) minimal media in exponential growth (OD600=0.3) and stationary phase (OD600=1.5). RNA samples from wild type or rpoS mutants were extracted using acidic hot phenol method and hybridized to Affymetrix E. coli Antisense Genome Array.
