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Sample GSM32403 Query DataSets for GSM32403
Status Public on Oct 23, 2004
Title Untreated Raw 264.7 cells 2 hr vs Lipopolysaccharide (LPS) and 2-Methyl-thio-ATP treated RAw 264.7 cells 2 hr. (set 1)
Sample type RNA
 
Channel 1
Source name Total RNA from untreated RAW 264.7 cells was labeled with Cyanine-3 (green).
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Total RNA from LPS (100ng/100pM LPS binding protein) and 2MA (500uM) treated RAW 264.7 cells was label with Cyanine-5 (red).
Organism Mus musculus
Extracted molecule total RNA
 
 
Description All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 2 hours.
All samples were gathered from mouse RAW 264.7 cells (macrophages). Test total RNA was extracted from Lipopolysaccharide(100ng/100pM LPS binding protein) and 2-Methyl-thio-ATP (500uM) treated RAW 264.7 cells cultured for 2 hours.
 
Submission date Oct 15, 2004
Last update date May 27, 2005
Contact name Sangdun Choi
E-mail(s) schoi@caltech.edu
Phone 626-395-8732
Fax 626-796-7066
Organization name California Institute of Technology
Department Biology
Lab Simon Lab
Street address 1200 E. California Blvd
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
 
Platform ID GPL254
Series (1)
GSE1863 Dual Ligand Effects

Data table header descriptions
ID_REF Feature number which correspond to the order of genes on the array
G_PROCESSED Processed Signal, after dye normalization and background subtraction, used for computing the log ratio.
G_MEAN Green Channel Mean Raw Intensity
G_MEDIAN Green Channel Median Raw Intensity
G_SD Green Channel Standard Deviation
G_BG_MEAN Green Channel Mean Background Level
G_BG_MEDIAN Green Channel Median Background Level
G_BG_SD Green Channel Background Standard Deviation
R_PROCESSED Processed Signal, after dye normalization and background subtraction, used for computing the log ratio.
R_MEAN Red Channel Mean Raw Intensity
R_MEDIAN Red Channel Median Raw Intensity
R_SD Red Channel Standard Deviation
R_BG_MEAN Red Channel Mean Background Level
R_BG_MEDIAN Red Channel Median Background Level
R_BG_SD Red Channel Background Standard Deviation
RATIO Log Ratio (base 10)
VALUE Processed Ratio (base 2) after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots.

Data table
ID_REF G_PROCESSED G_MEAN G_MEDIAN G_SD G_BG_MEAN G_BG_MEDIAN G_BG_SD R_PROCESSED R_MEAN R_MEDIAN R_SD R_BG_MEAN R_BG_MEDIAN R_BG_SD RATIO VALUE
1 102328 54867 54282.5 2675.87 430.709 426 17.3657 4794.53 2500.09 2451.5 244.021 304.331 303 11.1784 -1.32925 0
2 120.767 470.37 470 14.7574 416.473 417 7.8285 110.424 352.815 349 13.8676 301.904 302 11.8469 -0.0388865 0
3 17.9834 419.833 419 7.91073 415.007 415 7.64853 26.0074 310.417 309.5 11.4623 302.837 302 11.6089 0.160225 0
4 180.171 497.654 499 15.7504 414.262 413.5 7.1713 168.715 383.769 382 19.7227 304.156 304 11.4175 -0.0285303 0
5 16.9528 418.577 416.5 8.68181 414.946 415 7.16927 25.3422 314.308 316.5 14.2373 307.027 307 11.2504 0.174603 0
6 219.014 517.88 521 12.8463 415.275 415 7.7378 184.942 395.76 398 14.5209 308.506 308 11.7532 -0.0734374 0
7 17.3826 416.417 417.5 6.28951 417.342 417 7.0498 27.2029 316.042 314 14.1313 307.013 307 12.6068 0.194499 0
8 22817.3 12546.5 12447.5 594.608 415.814 415 7.24663 21924.3 10377.8 10346.5 686.155 305.8 306 12.5275 -0.0173398 -0.057601568779727962
9 720.339 786.76 785 31.4063 415.659 416 7.52728 547.108 554.48 549 33.4628 303.659 303 12.9218 -0.119464 -0.39685081792762383
10 341.441 577.462 575 22.1346 413.594 414 7.02762 239.631 414.577 415.5 27.0691 301.387 301 12.6858 -0.153772 -0.51081952700701938
11 182.496 500.577 501 10.5078 416.238 415 8.43435 155.998 377.269 377.5 16.1383 304.069 304 11.9517 -0.068134 -0.22633624881705552
12 96285.2 48089.7 47463 4538.01 423.248 422 9.49815 71894.4 35801.7 34963 5823.93 309.389 307 13.8157 -0.126865 -0.42143640775788516
13 300.983 560.577 559.5 9.2959 416.521 416 7.56804 261.468 425.846 430.5 17.5378 301.533 300 12.7628 -0.0611244 -0.20305086164313305
14 188.549 500.217 498 13.4771 412.667 412 7.38255 174.125 385.391 387 12.8408 303.176 303 12.6549 -0.0345628 -0.11481513635797291
15 49619.1 25769.4 25789 1465.56 419.271 418 9.50141 43804.6 21253.4 20658 1836.1 302.516 302 13.0997 -0.0541291 -0.17981297804096752
16 266.462 551.25 552.5 15.1119 415.019 416 8.18881 1539.53 1017.54 985 123.614 299.648 299 12.0889 0.761752 2.5304853701366383
17 424.885 631.48 633 20.1683 416.926 417 7.67683 651.52 605.52 604 36.1042 300.456 299 11.5381 0.185656 0.61673588238440813
18 20099 11075.6 11029 357.386 416.636 417 8.87118 27413.2 12930.5 12964 345.46 303.937 305 12.3358 0.134785 0.44774607826939306
19 135.18 478.667 478 15.0077 417.883 418 8.08935 111.063 353.852 354 12.633 302.8 304 11.6652 -0.0853425 -0.28350164843792469
20 54304.6 28115.8 28195 1341.59 424.281 421 17.4883 42919.6 20873.9 20930 935.203 307.414 305 14.3195 -0.102181 -0.33943793466368555

Total number of rows: 16281

Table truncated, full table size 2083 Kbytes.




Supplementary data files not provided
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