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Sample GSM325453 Query DataSets for GSM325453
Status Public on Feb 01, 2010
Title Gata3_F2
Sample type RNA
Source name Gata3 overexpression line
Organism Mus musculus
Characteristics R1 ES cells expressing human Gata3 T2ER fusion
Treatment protocol To induce transgene activity, cells were treated with induction medium (TS medium + 1 µg/mL tamoxifen (Sigma)). For microarray analysis, ES cells were seeded at densities of 2 x 105 cells per gelatinized 35-mm well in ES medium, switched to induction medium the following day, and fed daily for the indicated time period.
Growth protocol To create stably transformed lines, 1-3 x 107 ES cells were electroporated with 20-30 µg plasmid (pCAG-mCdx2ER-ires-puror (Niwa et al., 2005), pCAG-hCdx2ERT2-ires-puror, or pCAG-hGata3ERT2-ires-puror), and seeded on two 10 cm gelatinized plates. Cells were fed with plasmid selection medium (ES medium + 1.2 µg/mL puromycin) until colonies were visible by eye. Colonies were picked and individually expanded on gelatin.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A second clean up step was done using the RNEasy kit from QIAGEN according to manufactures instructions
Label biotin
Label protocol as per facility protocols
Hybridization protocol as per facility protocols
Scan protocol Gene Chips were scanned using the Hewlett-Packard Gene Array Scanner G2500A.
Description Gata3 overexpression line replicate 4
Data processing The data were analyzed with Expression Console (Affymetrix) using the quartile normalization and PLIER algorithm
Submission date Sep 30, 2008
Last update date Nov 23, 2009
Contact name Brian Joseph Cox
Organization name University of Toronto
Department Physiology
Lab Cox System Biology
Street address 1 King's College Circle, Rm 3360
City Toronto
State/province Ontario
ZIP/Postal code M5S1A8
Country Canada
Platform ID GPL1261
Series (2)
GSE12986 Expression of Cdx2 or Gata3 in R1 mouse embryonic stem cells
GSE12999 Gata3 acts alongside Cdx2 to promote trophoblast gene expression downstream of Tead4 during mouse development

Data table header descriptions
VALUE Quantification

Data table
AFFX-BioB-5_at 929.538
AFFX-BioB-M_at 1101.2
AFFX-BioB-3_at 779.178
AFFX-BioC-5_at 1626.6
AFFX-BioC-3_at 1627.53
AFFX-BioDn-5_at 3028.04
AFFX-BioDn-3_at 6263.17
AFFX-CreX-5_at 14409.9
AFFX-CreX-3_at 15087.6
AFFX-DapX-5_at 25.4046
AFFX-DapX-M_at 38.1715
AFFX-DapX-3_at 7.15316
AFFX-LysX-5_at 16.1675
AFFX-LysX-M_at 63.929
AFFX-LysX-3_at 18.2967
AFFX-PheX-5_at 24.9847
AFFX-PheX-M_at 108.592
AFFX-PheX-3_at 95.7892
AFFX-ThrX-5_at 39.8847
AFFX-ThrX-M_at 89.0423

Total number of rows: 45101

Table truncated, full table size 850 Kbytes.

Supplementary file Size Download File type/resource
GSM325453.CEL.gz 4.1 Mb (ftp)(http) CEL
GSM325453.chp.gz 325.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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