Animals homogenized in RNA extraction buffer, RNA extracted with Qiagen Rneasy Kit Digital Gene Expression: poly-A selected, DpnII digest and sequenced on Illumina Genome Analyzer according to manufacturer's instructions
Library strategy
RNA-Seq
Library source
transcriptomic
Library selection
cDNA
Instrument model
Illumina Genome Analyzer
Description
5×8-20
Data processing
identical tags were condensed into a set of unique tags and a count equivalent to the number of times a tag was observed was assigned to each unique tag. Counts were analyzed using a linear model.
