|
| Status |
Public on Jul 12, 2018 |
| Title |
S30um-1 |
| Sample type |
SRA |
| |
|
| Source name |
ovary granulosa cell
|
| Organism |
Equus asinus africanus |
| Characteristics |
treatment: 30 um Zearalenone
cell type: Granulosa cell
tissue: Ovary
|
| Treatment protocol |
ZEA was added to the cultured medium at final concentrations of the 10 µM or 30 µM, then the cells were incubated with ZEA for 72 h. The control and 10 μM ZEA group added the same dose of DMSO to 30 μM ZEA group for accuracy.
|
| Growth protocol |
Donkey granulosa cells (dGCs) were aspirated from the antral follicles using a 10 ml syringe
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the cultured GCs by the use of an RNAprep pure MicroKit in line with the manufacturer’s instruction (Aidlab, RN07, Beijing, China)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using HISAT2 2.1.0 with default parameters
Count gene expression using htseq with default parameters
Genome_build: ASM130575v1
Supplementary_files_format_and_content: tab-delimited text files include gene expression count for each Sample
|
| |
|
| Submission date |
Jul 11, 2018 |
| Last update date |
Jul 12, 2018 |
| Contact name |
Zhang Rui Qian |
| E-mail(s) |
zhangruiqianv@gmail.com
|
| Organization name |
Qingdao Agricultural University
|
| Street address |
Great Wall Road
|
| City |
QingDao |
| State/province |
ShanDong |
| ZIP/Postal code |
266109 |
| Country |
China |
| |
|
| Platform ID |
GPL25320 |
| Series (1) |
| GSE116950 |
Zearalenone exposure enhanced the expression of tumorigenesis genes in donkey granulosa cells via the PTEN/PI3K/AKT signaling pathway |
|
| Relations |
| BioSample |
SAMN09643638 |
| SRA |
SRX4381678 |
