|
| Status |
Public on Nov 21, 2008 |
| Title |
endogenous small RNA associated to human Argonaute 2 |
| Sample type |
SRA |
| |
|
| Source name |
HEK293 cells
|
| Organism |
Homo sapiens |
| Characteristics |
antibody: anti-Ago2 11A9
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Argonaute complexes were immunoprecipitated with beads coated with anti-Ago1 or anti-Ago2 complexes. RNA was isolated by adding 40 μg Proteinase K in 200 μl Proteinase K buffer (300 mM NaCl, 25 mM EDTA, 2% SDS, 200 mM Tris HCl pH 7.5) directly onto beads followed by Phenol/Chloroform extraction and Ethanol precipitation. Small RNA cloning was performed by Vertis Biotechnologie AG (Weihenstephan, Germany)
|
| |
|
| Library strategy |
RIP-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
454 GS 20 |
| |
|
| Description |
endogenous small RNA associated to human Argonaute 2
|
| Data processing |
Processed data file contains the reads with 5' and 3' adapters removed
|
| |
|
| Submission date |
Oct 27, 2008 |
| Last update date |
May 15, 2019 |
| Contact name |
Gunter Meister |
| E-mail(s) |
meister@biochem.mpg.de
|
| Organization name |
Max Planck Institute of Biochemistry
|
| Department |
Center for Integrated Protein Science Munich (CIPSM)
|
| Lab |
RNA Biology
|
| Street address |
Am Klopferspitz 18
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL9178 |
| Series (1) |
| GSE13370 |
High-throughput pyrosequencing of endogenous small RNAs associated to human Argonaute 1 & 2 |
|
| Relations |
| SRA |
SRX012409 |
| BioSample |
SAMN00004736 |
