|
| Status |
Public on Feb 18, 2019 |
| Title |
Lats1/Lats2 DKO replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Lats1F/F;Lats2F/F;Nestin-Cre
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E12.5
tissue: Cortex
genotype/variation: Lats1F/F;Lats2F/F;Nestin-Cre double knock-out
|
| Treatment protocol |
Samples were untreated
|
| Growth protocol |
Cortices of E12.5 mouse embryos were dissected in cold PBS prior to isolation of RNA. Each sample was derived from an independent embryo.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol (Thermo Fisher Scientific)
|
| Label |
biotin
|
| Label protocol |
Sense target cDNA was prepared from 100ng of total RNA using the Ambion® WT Expression Kit (Life Technologies P/N 4411973). cDNA was fragmented and biotin-labeled using the Affymetrix® GeneChip® WT Terminal Labeling Kit (Affymetrix P/N 900671).
|
| |
|
| Hybridization protocol |
Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Mouse Gene 2.0 ST GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expresson Console software v1.1.
|
| |
|
| Submission date |
Sep 13, 2018 |
| Last update date |
Feb 19, 2019 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE119935 |
The Hippo Pathway Prevents YAP/TAZ–Driven Hypertranscription and Controls Neural Progenitor Number [microarray] |
| GSE120016 |
The Hippo Pathway Prevents YAP/TAZ–Driven Hypertranscription and Controls Neural Progenitor Number |
|
