|
Status |
Public on Nov 01, 2010 |
Title |
CRC patiƫnt 39 |
Sample type |
genomic |
|
|
Source name |
Blood from patients with early-onset colorectal cancer
|
Organism |
Homo sapiens |
Characteristics |
age at diagnosis: 49 # fisrt degree rel.: 1 tumor location: Rectum
|
Extracted molecule |
genomic DNA |
Extraction protocol |
QiaAmp purification kit (Qiagen)
|
Label |
biotin
|
Label protocol |
Biotinylated DNA was prepared according to the standard Affymetrix protocol from two times 250 ng genomic DNA (Genome-Wide Human SNP Nsp/Sty 6.0 User Guide manual 702504 Rev.1, Affymetrix).
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|
|
Hybridization protocol |
Hybridizations were performed according to the standard Affymetrix protocol from two times 250 ng genomic DNA (Genome-Wide Human SNP Nsp/Sty 6.0 User Guide manual 702504 Rev.1, Affymetrix) using an Affymetrix hybridisation oven 640 and an Affymetrix Fluidic station 450.
|
Scan protocol |
Scanning performed according to the standard Affymetrix protocol from two times 250 ng genomic DNA ((Genome-Wide Human SNP Nsp/Sty 6.0 User Guide manual 702504 Rev.1, Affymetrix) using an Affymetrix scanner 3000.
|
Description |
germline DNA from suspected familial microsatellite-stable CRC
|
Data processing |
Genotyping information obtained using Affymetrix Genotyping Concole software (v2.1)
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|
|
Submission date |
Oct 31, 2008 |
Last update date |
Nov 30, 2010 |
Contact name |
Roland P. Kuiper |
E-mail(s) |
r.kuiper@antrg.umcn.nl, e.verwiel@antrg.umcn.nl
|
Phone |
+31243610868
|
Fax |
+31243668752
|
Organization name |
Radboud University Nijmegen Medical Centre
|
Department |
Human Genetics
|
Street address |
Geert Grooteplein 10
|
City |
Nijmegen |
ZIP/Postal code |
6525GA |
Country |
Netherlands |
|
|
Platform ID |
GPL6801 |
Series (1) |
GSE13429 |
Identification of candidate predisposing copy number variants in familial and early-onset colorectal cancer patients |
|