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Sample GSM34072 Query DataSets for GSM34072
Status Public on Jul 01, 2005
Title TGCT cell line 2102Ep/clone 2A6, untreated, undifferentiated
Sample type RNA
 
Channel 1
Source name TGCT cell line 2102Ep/clone 2A6, untreated, undifferentiated
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Universal Human Reference RNA (Stratagene)
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description Testicular germ cell tumor (embryonal carcinoma) cell line 2102Ep (clone 2A6). Embryonal carcinoma cell lines are generally pluripotent and share features with embryonic stem cells. However, the 2102Ep cell line is relatively nullipotent. Time point 0 days of culturing with retinoic acid (untreated). Maintained at high cell density (>5 000 000 cells per 75 cm2 tissue culture flask) in Dulbecco Modified Eagles Medium (DMEM high glucose formulation; GIBCO LifeTechnologies, UK) supplemented with 10% fetal calf serum.
Keywords = cell line
Keywords = differentiation
Keywords = embryonal carcinoma
Keywords = embryonic stem cell
Keywords = nullipotency
Keywords = nullipotent
Keywords = pluripotency
Keywords = pluripotent
Keywords = retinoic acid
Keywords = seminoma
Keywords = teratocarcinoma
Keywords = testicular germ cell tumor
Keywords = testicular neoplasm
Keywords = testis
Keywords = totipotency
Keywords = undifferentiated
 
Submission date Oct 29, 2004
Last update date Mar 09, 2006
Contact name Rolf I Skotheim
E-mail(s) rolf.i.skotheim@rr-research.no
Phone +47 2293 4000
Fax +47 2293 5767
URL http://www.radium.no/cancerprevention
Organization name Rikshospitalet-Radiumhospitalet Medical Center, Institute for Cancer Research
Department Department of Cancer Prevention
Street address
City Oslo
ZIP/Postal code N-0310
Country Norway
 
Platform ID GPL885
Series (1)
GSE1818 Testicular germ cell tumors and their histological subgroups

Data table header descriptions
ID_REF
FLAG Boolean for measurement quality. 1=Feature with inadequate measurements, 0=Feature with good quality measurements
VALUE same as UNF_VALUE but with flagged values removed
Log2_NormalTestisCenteredRatio For features with FLAG=0: Log2(SampleRATIO/MedianRATIO of the three Normal testis samples); gives an indication of up- and downregulation in the sample relative to the mRNA level in normal testis
RATIO For features with FLAG=0: CH1_ProcessedSignal / CH2_ProcessedSignal
PositionX X-coordinate, pixel position, of feature centroid on the original scan (tif-image)
PositionY Y-coordinate, pixel position, of feature centroid on the original scan (tif-image)
CH1_SurrogateUsed The CH1 surrogate value used
CH2_SurrogateUsed The CH2 surrogate value used
CH1_ProcessedSignal The propagated CH1 feature signal, used further for computation of ratios and log2ratios
CH2_ProcessedSignal The propagated CH2 feature signal, used further for computation of ratios and log2ratios
CH1_ProcessedSigError Standard error of propagated CH1 feature signal
CH2_ProcessedSigError Standard error of propagated CH2 feature signal
CH1_AREA Total number of pixels used to compute CH1 feature statistics
CH2_AREA Total number of pixels used to compute CH2 feature statistics
CH1_MEAN Raw mean signal of feature in CH1
CH2_MEAN Raw mean signal of feature in CH2
CH1_MEDIAN Raw median signal of feature in CH1
CH2_MEDIAN Raw median signal of feature in CH2
CH1_SD Standard deviation of all inlier pixels per CH1 feature
CH2_SD Standard deviation of all inlier pixels per CH2 feature
CH1_BKD_AREA Total number of pixels used to compute local CH1 background statistics per spot
CH2_BKD_AREA Total number of pixels used to compute local CH2 background statistics per spot
CH1_BKD_MEAN Mean local background CH1 signal (local to corresponding feature)
CH2_BKD_MEAN Mean local background CH2 signal (local to corresponding feature)
CH1_BKD_MEDIAN Median local background CH1 signal (local to corresponding feature)
CH2_BKD_MEDIAN Median local background CH2 signal (local to corresponding feature)
CH1_BKD_SD Standard deviation of all inlier pixels per local CH1 background of each feature
CH2_BKD_SD Standard deviation of all inlier pixels per local CH2 background of each feature
CH1_NumSatPix Total number of CH1 saturated pixels per feature
CH2_NumSatPix Total number of CH2 saturated pixels per feature
CH1_IsSaturated Boolean flag indicating if a feature is saturated for CH1 or not. 1=Saturated (>50% of the pixels above the saturation threshold), 0=NotSaturated
CH2_IsSaturated Boolean flag indicating if a feature is saturated for CH2 or not. 1=Saturated (>50% of the pixels above the saturation threshold), 0=NotSaturated
CH1_IsFeatNonUnifOL Boolean flag indicating if a feature is a NonUniformity Outlier or not for CH1. 1=The feature is non-uniform in that the pixel noise of feature exceeds a threshold established for a "uniform" feature, 0=Uniform feature
CH2_IsFeatNonUnifOL Boolean flag indicating if a feature is a NonUniformity Outlier or not for CH2. 1=The feature is non-uniform in that the pixel noise of feature exceeds a threshold established for a "uniform" feature, 0=Uniform feature
CH1_IsBGNonUnifOL Boolean flag indicating if a feature is a Background NonUniformity Outlier or not for CH1. 1=The background is non-uniform in that the pixel noise of feature background exceeds a threshold established for a "uniform" background, 0=Uniform background
CH2_IsBGNonUnifOL Boolean flag indicating if a feature is a Background NonUniformity Outlier or not for CH2. 1=The background is non-uniform in that the pixel noise of feature background exceeds a threshold established for a "uniform" background, 0=Uniform background
CH1_IsFeatPopnOL Boolean flag indicating if a feature is a Population Outlier or not for CH1. Probes with replicate features on the microarray were examined using population statistics. 1=IsPopulationOutlier, 0=IsNotPopulationOutlier
CH2_IsFeatPopnOL Boolean flag indicating if a feature is a Population Outlier or not for CH2. Probes with replicate features on the microarray were examined using population statistics. 1=IsPopulationOutlier, 0=IsNotPopulationOutlier
CH1_BGSubSignal The net CH1 signal following the subtraction of the CH1 background from the raw mean CH1 signal
CH2_BGSubSignal The net CH2 signal following the subtraction of the CH2 background from the raw mean CH2 signal
CH1_BGSubSigError Propagated standard error as computed on net CH1 background subtracted signal
CH2_BGSubSigError Propagated standard error as computed on net CH2 background subtracted signal
CH1_IsWellAboveBG Boolean flag indicating if CH1 for a feature is WellAbove Background or not. 1=WellAboveBackground, 0=NotWellAboveBackground
CH2_IsWellAboveBG Boolean flag indicating if CH2 for a feature is WellAbove Background or not. 1=WellAboveBackground, 0=NotWellAboveBackground
IsNormalization A boolean flag which indicates if a feature is used to measure dye bias. 1=Feature used, 2=Feature not used.
CH1_DyeNormSignal The CH1 dye normalized signal
CH2_DyeNormSignal The CH2 dye normalized signal
CH1_DyeNormError The standard error associated with the CH1 dye normalized signal
CH2_DyeNormError The standard error associated with the CH2 dye normalized signal
DyeNormCorrelation Dye normalized CH1 and CH2 pixel correlation
UNF_VALUE Expression level (mRNA abundance) relative to the common universal human reference RNA. Log2(CH1_ProcessedSignal/CH2_Processed signal)

Data table
ID_REF FLAG VALUE Log2_NormalTestisCenteredRatio RATIO PositionX PositionY CH1_SurrogateUsed CH2_SurrogateUsed CH1_ProcessedSignal CH2_ProcessedSignal CH1_ProcessedSigError CH2_ProcessedSigError CH1_AREA CH2_AREA CH1_MEAN CH2_MEAN CH1_MEDIAN CH2_MEDIAN CH1_SD CH2_SD CH1_BKD_AREA CH2_BKD_AREA CH1_BKD_MEAN CH2_BKD_MEAN CH1_BKD_MEDIAN CH2_BKD_MEDIAN CH1_BKD_SD CH2_BKD_SD CH1_NumSatPix CH2_NumSatPix CH1_IsSaturated CH2_IsSaturated CH1_IsFeatNonUnifOL CH2_IsFeatNonUnifOL CH1_IsBGNonUnifOL CH2_IsBGNonUnifOL CH1_IsFeatPopnOL CH2_IsFeatPopnOL CH1_BGSubSignal CH2_BGSubSignal CH1_BGSubSigError CH2_BGSubSigError CH1_IsWellAboveBG CH2_IsWellAboveBG IsNormalization CH1_DyeNormSignal CH2_DyeNormSignal CH1_DyeNormError CH2_DyeNormError DyeNormCorrelation UNF_VALUE
1 0 0.719336916 -1.834860115 1.64642514 49.5517 47.462 0 0 608.28 369.455 11.2209 8.02671 60 60 812.633 495.967 797 479.5 92.5058 70.3447 237 237 61.27 70.0549 60 70 7.27915 9.19614 0 0 0 0 0 0 0 0 0 0 647.9 418.907 11.9518 9.10108 1 1 0 608.28 369.455 11.2209 8.02671 0.782546 0.719336916
2 1 72.5375 45.2188 9.0904 0 9.0904 10.1607 2.49857 1.20008 55 55 149.127 93.6182 146 92 13.2246 13.8718 257 257 58.3774 69.8677 57 68 6.66157 10.302 0 0 0 0 0 0 0 0 0 0 -12.7137 16.7452 1.83098 1.97778 0 0 0 -17.3491 10.1607 2.49857 1.20008 -0.249056 -0.160584114
3 0 0.089499573 -0.418542248 1.064001049 92.4238 46.6318 0 0 267.808 251.699 5.02549 5.16555 49 49 443.02 372.061 441 372 36.6296 41.5383 263 263 59.9506 73.9049 59 72 6.23226 11.3536 0 0 0 0 0 0 0 0 0 0 279.606 291.151 5.24689 5.9752 1 1 1 267.808 251.699 5.02549 5.16555 0.490811 0.089499573
4 0 -0.117281853 -0.943757211 0.921922988 114.068 46.5903 0 0 34010.2 36890.5 482.653 629.436 60 60 36937.7 41294.6 36627.5 41316 4042 5446.3 258 258 64.1938 78.9031 63 77 8.11117 13.0206 0 0 0 0 0 0 0 0 0 0 36770.1 41208.7 521.82 703.114 1 1 1 34010.2 36890.5 482.653 629.436 0.994262 -0.117281853
5 0 -0.241138813 -0.520366269 0.846077185 135.543 47.6026 0 0 744.542 879.993 13.9252 13.9066 58 58 980 1048.98 979.5 1043.5 116.196 116.59 267 267 60.4382 72.3933 60 72 7.13346 10.4791 0 0 0 0 0 0 0 0 0 0 816.098 969.584 15.2635 15.3225 1 1 1 744.542 879.993 13.9252 13.9066 0.789959 -0.241138813
7 0 0.672118392 -2.367271173 1.593410947 178.099 46.2113 0 0 345.861 217.057 6.29811 5.00224 62 62 525.339 327.774 526.5 325.5 51.7978 45.2829 269 269 60.0781 70.1747 60 70 5.94294 8.66085 0 0 0 0 0 0 0 0 0 0 361.797 250.594 6.5883 5.77512 1 1 0 345.861 217.057 6.29811 5.00224 0.623939 0.672118392
8 0 2.606297516 2.954306956 6.089389163 199.409 45.9799 0 0 9870.23 1620.89 118.065 26.8941 60 60 11139.1 1839.33 11113 1815.5 1016.66 226.26 259 259 63.0425 71.4479 62 70 7.42626 10.1317 0 0 0 0 0 0 0 0 0 0 10972.6 1760.88 131.252 29.2169 1 1 1 9870.23 1620.89 118.065 26.8941 0.910132 2.606297516
9 0 -1.036303812 -1.402461785 0.487575043 219.257 47.674 0 0 375.542 770.224 5.6856 15.3247 56 56 577.982 949.464 578.5 943 45.0658 129.084 237 237 71.9916 74.616 68 73 14.3751 11.9306 0 0 0 0 0 0 0 0 0 0 402.527 867.843 6.09413 17.267 1 1 1 375.542 770.224 5.6856 15.3247 0.689014 -1.036303812
11 0 -0.134735867 -0.663298272 0.910836577 261.838 48.3659 0 0 363.626 399.222 7.77299 10.6904 60 60 548.483 534.083 538.5 525 63.5523 94.4795 256 256 60.5781 71.043 60 70 7.50436 9.50057 0 0 0 0 0 0 0 0 0 0 384.442 456.035 8.21797 12.2117 1 1 1 363.626 399.222 7.77299 10.6904 0.782185 -0.134735867
13 0 0.224578614 0.38322374 1.168435919 304.936 47.8761 0 0 422.774 361.829 9.33739 8.2132 63 63 610.587 491.492 614 490 78.32 74.1276 230 230 59.8 71.487 59 70 7.44728 12.3645 0 0 0 0 0 0 0 0 0 0 447.324 413 9.8796 9.37472 1 1 1 422.774 361.829 9.33739 8.2132 0.612296 0.224578614
14 0 0.582392807 -2.513458281 1.497330614 326.059 46.5074 0 0 368.529 246.124 5.8131 5.57901 58 58 549.224 361.379 552 364 46.3919 48.5996 263 263 58.8137 71.2776 58 70 6.21924 10.9518 0 0 0 0 0 0 0 0 0 0 386.947 283.096 6.10362 6.41708 1 1 0 368.529 246.124 5.8131 5.57901 0.625378 0.582392807
15 0 0.356847317 0.209225198 1.280624323 346.232 48.6984 0 0 3748.81 2927.33 57.4058 61.5767 61 61 4347.11 3252.1 4344 3194 500.057 520.713 245 245 62.4245 75.3714 62 73 7.42555 12.7593 0 0 0 0 0 0 0 0 0 0 4181.23 3169.72 64.0275 66.6754 1 1 1 3748.81 2927.33 57.4058 61.5767 0.945455 0.356847317
16 0 -0.32795764 -0.378352538 0.796663487 367.731 48.4214 0 0 918.792 1153.3 15.8711 16.824 59 59 1184.12 1332.42 1192 1339 135.493 140.295 255 255 59.2118 72.5529 59 72 6.46753 10.3989 0 0 0 0 0 0 0 0 0 0 1021.44 1252.87 17.6443 18.2765 1 1 1 918.792 1153.3 15.8711 16.824 0.740758 -0.32795764
17 0 0.086701712 -1.231843974 1.0619396 389.453 47.6237 0 0 393.798 370.829 9.15348 8.33219 58 58 580.328 505.207 583.5 508 73.6434 72.282 259 259 60.4865 74.6371 60 74 6.51495 11.3168 0 0 0 0 0 0 0 0 0 0 416.378 423.565 9.67832 9.51711 1 1 1 393.798 370.829 9.15348 8.33219 0.637756 0.086701712
21 0 0.688242722 -2.53265403 1.611319651 474.01 47.5799 0 0 369.163 229.106 7.31144 5.97715 58 58 550.897 343.983 541 341.5 58.3085 52.1995 265 265 60.3509 73.0943 60 72 6.38902 10.5066 0 0 0 0 0 0 0 0 0 0 387.082 263.883 7.66634 6.88445 1 1 0 369.163 229.106 7.31144 5.97715 0.635636 0.688242722
23 0 -0.088846247 1.06850813 0.940274406 516.375 48.625 0 0 2509.63 2669.04 41.9369 45.1713 58 58 2972.48 2970.64 3011.5 2923 356.718 371.922 264 264 65.8712 77.75 65 76.5 7.63562 11.8706 0 0 0 0 0 0 0 0 0 0 2803.15 2885.88 46.8417 48.8412 1 1 1 2509.63 2669.04 41.9369 45.1713 0.929017 -0.088846247
25 0 -0.333343859 -0.289951052 0.793694735 559 48.5 0 0 1078.02 1358.23 16.2142 20.3325 60 60 1367.92 1547.55 1370 1537.5 140.224 170.259 277 277 60.5704 71.6968 60 70 6.58002 9.893 0 0 0 0 0 0 0 0 0 0 1203.88 1468.85 18.1072 21.9884 1 1 1 1078.02 1358.23 16.2142 20.3325 0.829297 -0.333343859
26 0 -0.676150227 1.12423239 0.625833058 580.416 47.6256 0 0 322.473 515.27 6.32446 11.1328 58 58 505.155 669.017 500 671 50.9313 96.4866 259 259 60.0463 74.6409 59 73 6.68373 11.8088 0 0 0 0 0 0 0 0 0 0 341.645 587.371 6.70048 12.6905 1 1 1 322.473 515.27 6.32446 11.1328 0.640603 -0.676150227
28 0 0.719620005 -3.162960553 1.646748236 622.935 47.7696 0 0 376.445 228.599 7.57347 6.60633 61 61 558.459 343.377 570 345 61.962 59.1715 262 262 60.1412 73.1641 59 72 6.57983 10.9904 0 0 0 0 0 0 0 0 0 0 394.854 263.208 7.94383 7.60651 1 1 0 376.445 228.599 7.57347 6.60633 0.523294 0.719620005
29 0 0.953007636 -1.743526739 1.9359043 643.408 48.708 0 0 506.208 261.484 10.6643 5.46402 59 59 697.695 377.424 694 371 86.4472 47.7216 257 257 59.7471 71.3307 59 70 5.64391 10.8816 0 0 0 0 0 0 0 0 0 0 534.484 299.088 11.26 6.24979 1 1 1 506.208 261.484 10.6643 5.46402 0.540341 0.953007636

Total number of rows: 19061

Table truncated, full table size 5558 Kbytes.




Supplementary file Size Download File type/resource
GSM34072.tif.gz 24.7 Mb (ftp)(http) TIFF

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